JoVE Encyclopedia of Experiments
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Overview
Encyclopedia of Experiments
This video demonstrates an in vitro technique to measure the cytotoxic ability of cytokine-induced killer (CIK) cells against cancer cells. Upon co-culturing target cancer cells with CIK cells, the dead cancer cells are identified using a fluorescent viability dye and are quantified using flow cytometry, estimating the cytotoxic effect of CIK cells.
This article has been published
Video Coming Soon
Source: Hsiao, C. H., et al. Isolation and Expansion of Cytotoxic Cytokine-induced Killer T Cells for Cancer Treatment. J. Vis. Exp. (2020).
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved