JoVE Encyclopedia of Experiments
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Encyclopedia of Experiments
This video demonstrates the staining and preparation of bacterial cells for immunological studies using red fluorescent dye. The stained bacteria are diluted and resuspended in a growth medium to achieve the desired bacterial concentration for host cell infection, representing the multiplicity of infection.
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Source: Yang, J. et al., Automated, High-Throughput Detection of Bacterial Adherence to Host Cells. J. Vis. Exp. (2021)
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