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Encyclopedia of Experiments

Analyzing Basophil Activation in the Presence of a Test Allergen using Flow Cytometry

In this video, we demonstrate the test to evaluate basophil activation in the presence of a test allergen using flow cytometry.

All procedures involving sample collection have been performed in accordance with the institute's IRB guidelines.

1. Sample preparation

  1. Collect peripheral blood in 9 mL heparinized tubes and maintain the sample at room temperature (RT) in a rotor until it is required for the experimental protocol.
  2. Label 5 mL cytometer tubes for negative controls (2 tubes), positive controls (2 tubes), and different concentrations of allergen/drug (1 t.......

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Source: Doña, I. et al., Basophil Activation Test for Allergy Diagnosis. J. Vis. Exp. (2021)

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