JoVE Encyclopedia of Experiments
Encyclopedia of Experiments
This video demonstrates label-free identification of lymphocyte morphology and biochemistry through 3D quantitative phase imaging. This technique allows for a thorough analysis of lymphocytes, uncovering their internal structures and properties without the need for labeling procedures.
1. Flow Cytometry and Sorting of Lymphocyte Subtypes
NOTE: Sorting lymphocytes depending on cell type is essential for establishing the ground-truth (i.e., correct) cell type labels to train and test a cell type classifier in supervised learning. Flow cytometry, a gold standard method, is used to identify and separate lymphocytes.
This article has been published
Video Coming Soon
Source: Yoon, J. et al. Label-Free Identification of Lymphocyte Subtypes Using Three-Dimensional Quantitative Phase Imaging and Machine Learning. J. Vis. Exp. (2018)
Copyright © 2024 MyJoVE Corporation. All rights reserved