JoVE Encyclopedia of Experiments
Encyclopedia of Experiments
This video demonstrates mast cell exocytosis induced by an activating reagent that includes a calcium ionophore, initiating the release of secretory granules containing the fluorescent reporter protein NPY-mRFP. Subsequently, we quantitatively assess the exocytosis by measuring the released NPY-mRFP using a fluorescence plate reader.
1. Measuring NPY-mRFP Exocytosis
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Source: Azouz, N. P., et al., Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis. J. Vis. Exp. (2015)
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