JoVE Encyclopedia of Experiments
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Encyclopedia of Experiments
A Fluorescence-Based Assay Using Engineered Lymphocytes for Screening Immunomodulatory Compounds
Overview
This video demonstrates the screening of immunomodulatory compounds via fluorescent-based assay. Transgenic mouse-derived T lymphocytes with green fluorescent protein expression cassettes are treated with these compounds. The enhanced and reduced GFP signals enable the identification of the compounds' stimulatory and inhibitory effects, respectively.
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Source: Fouda, A., et al., A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules. J. Vis. Exp., (2017).
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