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Encyclopedia of Experiments

An In Vitro Technique to Study T Cell Interaction with Supported Planar Lipid Bilayers

This video demonstrates an in vitro technique to study immune synapse formation between primary human T cells and supported lipid bilayers (SLB). T cell surface proteins interact with respective ligands immobilized on SLB to form an immune synapse, followed by the movement of lytic granules towards the synapse, which is visualized using fluorescence microscopy.

All procedures involving sample collection have been performed in accordance with the institute's IRB guidelines.

1. Isolation of CD4+ T Cells for an Image Analysis

  1. Thaw a 1 mL aliquot containing 107 frozen peripheral blood mononuclear cells (PBMCs) or lymph node mononuclear cells (LNMCs) from collected samples. In a sterile hood, add the thawed cells to 9 mL of RPMI supplemented with penicillin/streptomycin and glutamine.
    1. Cent.......

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