JoVE Encyclopedia of Experiments
Encyclopedia of Experiments
This video demonstrates an in vitro technique to study immune synapse formation between primary human T cells and supported lipid bilayers (SLB). T cell surface proteins interact with respective ligands immobilized on SLB to form an immune synapse, followed by the movement of lytic granules towards the synapse, which is visualized using fluorescence microscopy.
All procedures involving sample collection have been performed in accordance with the institute's IRB guidelines.
1. Isolation of CD4+ T Cells for an Image Analysis
This article has been published
Video Coming Soon
Source: Steblyanko, M., et al. Assessment of the Synaptic Interface of Primary Human T Cells from Peripheral Blood and Lymphoid Tissue. J. Vis. Exp. (2018).
Copyright © 2024 MyJoVE Corporation. All rights reserved