JoVE Encyclopedia of Experiments
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Encyclopedia of Experiments
This video demonstrates an in vitro technique to study immune synapse formation between primary human T cells and supported lipid bilayers (SLB). T cell surface proteins interact with respective ligands immobilized on SLB to form an immune synapse, followed by the movement of lytic granules towards the synapse, which is visualized using fluorescence microscopy.
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Source: Steblyanko, M., et al. Assessment of the Synaptic Interface of Primary Human T Cells from Peripheral Blood and Lymphoid Tissue. J. Vis. Exp. (2018).
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