Bacterial Culture Filtrate Protein Concentration using an Ultrafiltration Device

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Encyclopedia of Experiments

Bacterial Culture Filtrate Protein Concentration using an Ultrafiltration Device

Overview

This video demonstrates the isolation and concentration of bacterial culture filtrate proteins, which include bacterial extracellular vesicles, using ultrafiltration. The concentrated extracellular vesicles serve as diagnostic markers for disease detection.

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Source: Ryan, J. M., et al. Mycobacterium tuberculosis Extracellular Vesicle Enrichment through Size Exclusion Chromatography. J. Vis. Exp. (183) (2022)