A subscription to JoVE is required to view this content. Sign in or start your free trial.
Abstract
Immunology and Infection
The article describes a readily easy adaptive in vitro model to investigate macrophage polarization. In the presence of GM-CSF/M-CSF, hematopoietic stem/progenitor cells from the bone marrow are directed into monocytic differentiation, followed by M1 or M2 stimulation. The activation status can be tracked by changes in cell surface antigens, gene expression and cell signaling pathways.
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved