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Abstract

Biology

制备胰腺腺泡细胞钙成像的目的,细胞损伤的测量,以及腺病毒感染

Published: July 5th, 2013

DOI:

10.3791/50391

1Rangos Research Center, Pediatric Gastroenterology, Hepatology, and Nutrition, Children's Hospital of Pittsburgh of UPMC, 2Department of Surgery, Tufts University Medical Center

胰腺外分泌胰腺细胞是主要的实质细胞,进入胰管和胰腺酶的分泌起着主要作用。这也是网站发起胰腺炎。在这里,我们介绍了如何腺泡细胞分离从整个胰腺组织及细胞内钙信号的测量。此外,我们描述的技术,与腺病毒构建体转染这些细胞,并随后测量乳酸脱氢酶,细胞损伤的标志物的泄漏,以及在体外诱导腺泡细胞损伤的条件。这些技术提供了强大的工具来表征腺泡细胞生理和病理。

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