使用开放式现场迷宫来衡量小鼠的运动能力和焦虑样行为

Summary

A protocol is provided to use an Open Field Maze to access general locomotor activity, anxiety and emotionality in a laboratory mouse model.

Abstract

Animal models have proven to be invaluable to researchers trying to answer questions regarding the mechanisms of behavior. The Open Field Maze is one of the most commonly used platforms to measure behaviors in animal models. It is a fast and relatively easy test that provides a variety of behavioral information ranging from general ambulatory ability to data regarding the emotionality of the subject animal. As it relates to rodent models, the procedure allows the study of different strains of mice or rats both laboratory bred and wild-captured. The technique also readily lends itself to the investigation of different pharmacological compounds for anxiolytic or anxiogenic effects. Here, a protocol for use of the open field maze to describe mouse behaviors is detailed and a simple analysis of general locomotor ability and anxiety-related emotional behaviors between two strains of C57BL/6 mice is performed. Briefly, using the described protocol we show Wild Type mice exhibited significantly less anxiety related behaviors than did age-matched Knock Out mice while both strains exhibited similar ambulatory ability.

Introduction

The Open Field Maze (OFM) was initially developed in 1934 as a test to measure emotionality in rodents¹. It has attained the status of being one of the most widely used measures of behavior in animal psychology². It provides an easy and fairly rapid assessment of well-defined behaviors requiring no training to the test subject and little to no specialized training for the human administering the test. These attributes have led to wide-spread use of the open field maze in research extended to other animal species such as calves, pigs, rabbits, primates, honeybees and lobsters³. Part of its popularity arises from the fact that the psycho....

Protocol

注：此处进行的所有手术均由IACUC（研究合规办公室）提交给批准，并进行以下NIH的指导方针。在行为测试模式用于小鼠幼稚，不用于其他测试。的C57BL / 6野生型和敲除小鼠在此协议中使用先前已被描述^图9和这里介绍的数据是从该手稿。

1.准备的检测室和开放现场设备

1. 使用多单元旷场迷宫（OFM）包括四个活动室中被用于此分析（ 图1）。每个腔室?.......

Discussion

旷场迷宫是在动物行为学研究中最广泛使用的平台之一。一些重要的常规和行为学参数^2,4可以收集和OFM的表现时进行分析。这些数据允许研究者测量行为，从整体运动活性焦虑有关的情感行为^8。然而，使用OFM的也不是没有缺点。一个混杂的问题是大范围可以在任何测试期间被操纵的静态变量。例子包括时间，照明条件和新的物体包容。变异性在实验方案的设置和设计，这是必要?.......

Materials

Name	Company	Catalog Number	Comments
Multi Unit Open Field Test	San Diego Instruments, Inc.	White 7001-0354	Any single or multi unit open field maze can be used
SMART DT Tracking Software	PanLab/Harvard Apparatus	76-0695	Any tracking software can be utilized with this protocol
Sony 990x Video Camera Recorder	Sony	CCD-TRV328	Any suitable video camera can be attached to computer for recording tracking profiles.