Het gebruik van het open veld Labyrint aan Locomotorische en angst-achtig gedrag in Muizen Meet

Summary

A protocol is provided to use an Open Field Maze to access general locomotor activity, anxiety and emotionality in a laboratory mouse model.

Abstract

Animal models have proven to be invaluable to researchers trying to answer questions regarding the mechanisms of behavior. The Open Field Maze is one of the most commonly used platforms to measure behaviors in animal models. It is a fast and relatively easy test that provides a variety of behavioral information ranging from general ambulatory ability to data regarding the emotionality of the subject animal. As it relates to rodent models, the procedure allows the study of different strains of mice or rats both laboratory bred and wild-captured. The technique also readily lends itself to the investigation of different pharmacological compounds for anxiolytic or anxiogenic effects. Here, a protocol for use of the open field maze to describe mouse behaviors is detailed and a simple analysis of general locomotor ability and anxiety-related emotional behaviors between two strains of C57BL/6 mice is performed. Briefly, using the described protocol we show Wild Type mice exhibited significantly less anxiety related behaviors than did age-matched Knock Out mice while both strains exhibited similar ambulatory ability.

Introduction

The Open Field Maze (OFM) was initially developed in 1934 as a test to measure emotionality in rodents¹. It has attained the status of being one of the most widely used measures of behavior in animal psychology². It provides an easy and fairly rapid assessment of well-defined behaviors requiring no training to the test subject and little to no specialized training for the human administering the test. These attributes have led to wide-spread use of the open field maze in research extended to other animal species such as calves, pigs, rabbits, primates, honeybees and lobsters³. Part of its popularity arises from the fact that the psycho....

Protocol

OPMERKING: Alle procedures hier uitgevoerd werden door IACUC (Office of Research Compliance) ingediend bij en goedgekeurd en werden uitgevoerd na NIH richtlijnen. Muizen gebruikt in de gedrags-testen paradigma waren naïef en niet gebruikt voor andere tests. De C57BL / 6 Wild Type en Knock Out muizen gebruikt in dit protocol zijn eerder beschreven ⁹ en de hier gepresenteerde gegevens zijn afkomstig uit dat manuscript.

1. Voorbereiding van de testkamer en Open Gebied Apparatus

Discussion

Het open veld Maze is een van de meest gebruikte platforms in dierlijke gedragsonderzoek. Een aantal belangrijke conventionele en ethologische parameters ^2,4 kunnen worden verzameld en tijdens de uitvoering van de OFM geanalyseerd. Deze gegevens maken het mogelijk de onderzoeker om gedrag, variërend van algemene bewegingsactiviteit om angst-gerelateerde emotionele gedrag ⁸ meten. Echter, het gebruik van OSM is niet zonder gebreken. Een verwarrende kwestie is de brede waaier van statische variabele.......

Materials

Name	Company	Catalog Number	Comments
Multi Unit Open Field Test	San Diego Instruments, Inc.	White 7001-0354	Any single or multi unit open field maze can be used
SMART DT Tracking Software	PanLab/Harvard Apparatus	76-0695	Any tracking software can be utilized with this protocol
Sony 990x Video Camera Recorder	Sony	CCD-TRV328	Any suitable video camera can be attached to computer for recording tracking profiles.