Anvendelse af Open Field Maze til Mål Lokomotorisk og Angst-lignende opførsel i mus

Summary

A protocol is provided to use an Open Field Maze to access general locomotor activity, anxiety and emotionality in a laboratory mouse model.

Abstract

Animal models have proven to be invaluable to researchers trying to answer questions regarding the mechanisms of behavior. The Open Field Maze is one of the most commonly used platforms to measure behaviors in animal models. It is a fast and relatively easy test that provides a variety of behavioral information ranging from general ambulatory ability to data regarding the emotionality of the subject animal. As it relates to rodent models, the procedure allows the study of different strains of mice or rats both laboratory bred and wild-captured. The technique also readily lends itself to the investigation of different pharmacological compounds for anxiolytic or anxiogenic effects. Here, a protocol for use of the open field maze to describe mouse behaviors is detailed and a simple analysis of general locomotor ability and anxiety-related emotional behaviors between two strains of C57BL/6 mice is performed. Briefly, using the described protocol we show Wild Type mice exhibited significantly less anxiety related behaviors than did age-matched Knock Out mice while both strains exhibited similar ambulatory ability.

Introduction

The Open Field Maze (OFM) was initially developed in 1934 as a test to measure emotionality in rodents¹. It has attained the status of being one of the most widely used measures of behavior in animal psychology². It provides an easy and fairly rapid assessment of well-defined behaviors requiring no training to the test subject and little to no specialized training for the human administering the test. These attributes have led to wide-spread use of the open field maze in research extended to other animal species such as calves, pigs, rabbits, primates, honeybees and lobsters³. Part of its popularity arises from the fact that the psycho....

Protocol

BEMÆRK: Alle procedurer udføres her blev forelagt for og godkendt af IACUC (Office of Research Compliance) og blev gennemført efter NIH retningslinjer. Mus, der anvendes i den adfærdsmæssige test paradigme var naiv og ikke anvendes til andre tests. Den C57BL / 6 vildtype og Knock Out mus anvendes i denne protokol er blevet beskrevet tidligere ⁹ og data her, er fra dette manuskript.

1. Forberedelse af Test Room og åben mark Apparatur

1. Brug en multipel enhed åben ma.......

Discussion

Marken Maze er en af ​​de mest udbredte platforme i Dyreadfærdsundersøgelser. En række vigtige konventionelle og etologiske parametre ^2,4 kan indsamles og analyseres under udførelsen af OFM. Disse data gør det muligt for forskeren at måle adfærd lige fra overordnet bevægelsesaktivitet til angst-relaterede følelsesmæssige adfærd ^8. Men brug af OFM er ikke uden mangler. En confounding spørgsmål er den brede vifte af statiske variable, som kan manipuleres i løbet af en testsession. E.......

Materials

Name	Company	Catalog Number	Comments
Multi Unit Open Field Test	San Diego Instruments, Inc.	White 7001-0354	Any single or multi unit open field maze can be used
SMART DT Tracking Software	PanLab/Harvard Apparatus	76-0695	Any tracking software can be utilized with this protocol
Sony 990x Video Camera Recorder	Sony	CCD-TRV328	Any suitable video camera can be attached to computer for recording tracking profiles.