열기 필드 미로의 사용은 마우스의 운동력과 불안 같은 행동을 측정하기 위해

Summary

A protocol is provided to use an Open Field Maze to access general locomotor activity, anxiety and emotionality in a laboratory mouse model.

Abstract

Animal models have proven to be invaluable to researchers trying to answer questions regarding the mechanisms of behavior. The Open Field Maze is one of the most commonly used platforms to measure behaviors in animal models. It is a fast and relatively easy test that provides a variety of behavioral information ranging from general ambulatory ability to data regarding the emotionality of the subject animal. As it relates to rodent models, the procedure allows the study of different strains of mice or rats both laboratory bred and wild-captured. The technique also readily lends itself to the investigation of different pharmacological compounds for anxiolytic or anxiogenic effects. Here, a protocol for use of the open field maze to describe mouse behaviors is detailed and a simple analysis of general locomotor ability and anxiety-related emotional behaviors between two strains of C57BL/6 mice is performed. Briefly, using the described protocol we show Wild Type mice exhibited significantly less anxiety related behaviors than did age-matched Knock Out mice while both strains exhibited similar ambulatory ability.

Introduction

The Open Field Maze (OFM) was initially developed in 1934 as a test to measure emotionality in rodents¹. It has attained the status of being one of the most widely used measures of behavior in animal psychology². It provides an easy and fairly rapid assessment of well-defined behaviors requiring no training to the test subject and little to no specialized training for the human administering the test. These attributes have led to wide-spread use of the open field maze in research extended to other animal species such as calves, pigs, rabbits, primates, honeybees and lobsters³. Part of its popularity arises from the fact that the psycho....

Protocol

참고 : 여기서의 모든 절차에 제출하여 승인을 IACUC (연구 준수 사무소)에 의해 NIH의 지침에 따라 실시 하였다되었다. 행동 테스트 패러다임에 사용 된 마우스는 순진하고 다른 시험에 사용되지 않았다. 이 프로토콜에 사용되는 C57BL / 6 야생형과 똑 마우스는 ⁹ 이전에 설명하고 여기에 제시된 데이터는 원고에서 있습니다.

테스트 룸과 오픈 필드 장치 1. 준비

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Discussion

열기 필드 미로 동물 행동 연구에서 가장 널리 사용되는 플랫폼 중 하나입니다. 중요하고, 종래의 ^2,4- ethological 파라미터 번호를 수집하여 OFM의 수행 동안 분석 될 수있다. 이 자료는 연구자가 불안 관련 감정적 인 행동 ⁸ 전체 운동 활성에 이르기까지 행동을 측정 할 수 있습니다. 그러나, OFM의 사용에도 단점은 있습니다. 한가지 문제는 어떤 교란 시험 세션 동안 조작 될 수있는 정.......

Materials

Name	Company	Catalog Number	Comments
Multi Unit Open Field Test	San Diego Instruments, Inc.	White 7001-0354	Any single or multi unit open field maze can be used
SMART DT Tracking Software	PanLab/Harvard Apparatus	76-0695	Any tracking software can be utilized with this protocol
Sony 990x Video Camera Recorder	Sony	CCD-TRV328	Any suitable video camera can be attached to computer for recording tracking profiles.