Bruk av det åpne feltet Maze å måle Locomotor og angst-lignende oppførsel i Mus

Summary

A protocol is provided to use an Open Field Maze to access general locomotor activity, anxiety and emotionality in a laboratory mouse model.

Abstract

Animal models have proven to be invaluable to researchers trying to answer questions regarding the mechanisms of behavior. The Open Field Maze is one of the most commonly used platforms to measure behaviors in animal models. It is a fast and relatively easy test that provides a variety of behavioral information ranging from general ambulatory ability to data regarding the emotionality of the subject animal. As it relates to rodent models, the procedure allows the study of different strains of mice or rats both laboratory bred and wild-captured. The technique also readily lends itself to the investigation of different pharmacological compounds for anxiolytic or anxiogenic effects. Here, a protocol for use of the open field maze to describe mouse behaviors is detailed and a simple analysis of general locomotor ability and anxiety-related emotional behaviors between two strains of C57BL/6 mice is performed. Briefly, using the described protocol we show Wild Type mice exhibited significantly less anxiety related behaviors than did age-matched Knock Out mice while both strains exhibited similar ambulatory ability.

Introduction

The Open Field Maze (OFM) was initially developed in 1934 as a test to measure emotionality in rodents¹. It has attained the status of being one of the most widely used measures of behavior in animal psychology². It provides an easy and fairly rapid assessment of well-defined behaviors requiring no training to the test subject and little to no specialized training for the human administering the test. These attributes have led to wide-spread use of the open field maze in research extended to other animal species such as calves, pigs, rabbits, primates, honeybees and lobsters³. Part of its popularity arises from the fact that the psycho....

Protocol

MERK: Alle prosedyrer utført her ble forelagt og godkjent av IACUC (Office of Forskning Compliance) og ble utført følgende NIH retningslinjer. Mus brukes i atferds testing paradigmet var naiv og ikke brukes til andre tester. C57BL / 6 Wild Type og Knock Out mus som brukes i denne protokollen er blitt beskrevet tidligere ⁹ og dataene som presenteres her er fra det manuskriptet.

1. Klargjøring av Testing rommet og Open Feltet Apparatus

1. Bruke en multippel enhet åpent .......

Discussion

The Open Feltet Maze er en av de mest brukte plattformene i dyreatferdsstudier. En rekke viktige konvensjonelle og etologiske parametre ^2,4 kan samles inn og analyseres under utførelsen av OFM. Disse dataene tillater forskeren å måle atferd som spenner fra generelle bevegelsesaktivitet til angstrelaterte emosjonelle atferd ^8. Imidlertid er bruk av OFM ikke uten sine mangler. En forvirrende sak er bredt spekter av statiske variabler som kan manipuleres under enhver testøkt. Eksempler inkluderer .......

Materials

Name	Company	Catalog Number	Comments
Multi Unit Open Field Test	San Diego Instruments, Inc.	White 7001-0354	Any single or multi unit open field maze can be used
SMART DT Tracking Software	PanLab/Harvard Apparatus	76-0695	Any tracking software can be utilized with this protocol
Sony 990x Video Camera Recorder	Sony	CCD-TRV328	Any suitable video camera can be attached to computer for recording tracking profiles.