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Abstract

Developmental Biology

Generating Primary Fibroblast Cultures from Mouse Ear and Tail Tissues

Published: January 10th, 2016

DOI:

10.3791/53565

1Immunology Program, Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, 2NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore

Primary cells are derived directly from tissue and are thought to be more representative of the physiological state of cells in vivo than established cell lines. However, primary cell cultures usually have a finite life span and need to be frequently re-established. Fibroblasts are an easily accessible source of primary cells. Here, we discuss a simple and quick experimental procedure to establish primary fibroblast cultures from ears and tails of mice. The protocol can be used to establish primary fibroblast cultures from ears stored at RT for up to 10 days. When the protocol is carefully followed, contaminations are unlikely to occur despite the use of non-sterile tissue stored for extended time in some cases. Fibroblasts proliferate rapidly in culture and can be expanded to substantial numbers before undergoing replicative senescence.

Tags

Primary Fibroblast Cultures

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