Published: February 6th, 2019
This article presents a noninvasive biomonitoring system for the continuous recording and analyses of crayfish cardiac and locomotor activities. This system consists of a near-infrared optical sensor, a video-tracking module, and software for evaluating crayfish heartbeats that reflects its physiological condition and characterizes crayfish behavior during heartbeat fluctuations.
A crayfish is a pivotal aquatic organism that serves both as a practical biological model for behavioral and physiological studies of invertebrates and as a useful biological indicator of water quality. Even though crayfish cannot directly specify the substances that cause water quality deterioration, they can immediately (within a few seconds) warn humans of water quality deterioration via acute changes in their cardiac and behavioral activities.
In this study, we present a noninvasive method that is simple enough to be implemented under various conditions due to a combination of simplicity and reliability in one model.
This approach, in which the biological organisms are implemented into environmental evaluation processes, provides a reliable and timely alarm for warning of and preventing acute water deterioration in an ambient environment. Therefore, this noninvasive system based on crayfish physiological and ethological parameter recordings was investigated for the detection of changes in an aquatic environment. This system is now applied at a local brewery for controlling quality of the water used for beverage production, but it can be used at any water treatment and supply facility for continuous, real-time water quality evaluation and for regular laboratory investigations of crayfish cardiac physiology and behavior.
The subject of aquatic organisms' applications, both as model organisms for various laboratory investigations1,2 and as tools for monitoring industrial and natural/environmental water quality3,4, appears to be well studied. Nevertheless, this topic is still of noteworthy interest for humans, irrespective of whether they belong to the scientific community or to other occupations. In spite of the existence of a number of advanced methods for monitoring certain parameters (so-called "biomarkers")5,
1. Crayfish Selection
As a result, we obtained a combination of crayfish cardiac and behavioral activities, recorded and saved in a txt-format file (Figure 3). Besides the number of experimental crayfish, the date, and the sampling rate, the file consists of three columns: (1) the continual time in hh:mm:ss format; (2) the heart rate automatically calculated in beats per minute; (3) the locomotion registered as absence (0) or presence (1) of any movement. When the crayfish was ina.......
It has been widely suggested that the measurement of certain physiological parameters (such as heart or ventilation rate or both) is a more reliable method for recording crayfish reactions than the evaluation of behavioral responses that do not always occur immediately11. However, it is evident that the most efficient approach for assessing real crayfish reactions to environmental changes is the combination of cardiac activity and behavior recordings since that makes it possible to see the reason(.......
This study was supported by the Ministry of Education, Youth and Sports of the Czech Republic-projects "CENAKVA" No. CZ.1.05/2.1.00/01.0024 and "CENAKVA II'' No. LO1205 under the National Sustainability Program I, by the Grant Agency of the University of South Bohemia in České Budějovice (012/2016/Z), and by the Grant Agency of the Czech Republic (No. 16-06498S)....
|IR LED diode
|FTP KAT.5E 4X2X0,14C
|Kinect video camera
|University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, Institute of Complex Systems
|Link to the software: www.frov.jcu.cz/crayfishmonitoring
User name: frov
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