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Abstract

Chemistry

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

Published: January 19th, 2019

DOI:

10.3791/58771

1Molecular Diagnostics, Centre for Health and Bioresources, AIT Austrian Institute of Technology GmbH

Abstract

DNA origami nanostructures hold an immense potential to be used for biological and medical applications. However, low-salt conditions and nucleases in physiological fluids induce denaturation and degradation of self-assembled DNA nanostructures. In non-viral gene delivery, enzymatic degradation of DNA is overcome by the encapsulation of the negatively charged DNA in a cationic shell. Herein, inspired by gene delivery advancements, a simple, one-step and robust methodology is presented for the stabilization of DNA origami nanostructures by coating them with chitosan and linear polyethyleneimine. The polycation coating efficiently protects DNA origami nanostructures in Mg-depleted and nuclease-rich media. This method also preserves the full addressability of enzyme- and aptamer-based functionalization of DNA nanostructures.

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Keywords DNA Origami

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