Modeling the Size Spectrum for Macroinvertebrates and Fishes in Stream Ecosystems

Summary

This is a protocol to model the size spectrum (scaling relationship between individual mass and population density) for combined fish and invertebrate data from wadable streams and rivers. Methods include: field techniques to collect quantitative fish and invertebrate samples; lab methods to standardize the field data; and statistical data analysis.

Abstract

The size spectrum is an inverse, allometric scaling relationship between average body mass (M) and the density (D) of individuals within an ecological community or food web. Importantly, the size spectrum assumes that individual size, rather than species’ behavioral or life history characteristics, is the primary determinant of abundance within an ecosystem. Thus, unlike traditional allometric relationships that focus on species-level data (e.g., mean species’ body size vs. population density), size spectra analyses are ‘ataxic’ – individual specimens are identified only by their size, without consideration of taxonomic identity. Size spectra models are efficient representations of traditional, complex food webs and can be used in descriptive as well as predictive contexts (e.g., predicting responses of large consumers to changes in basal resources). Empirical studies from diverse aquatic ecosystems have also reported moderate to high levels of similarity in size spectra slopes, suggesting that common processes may regulate the abundances of small and large organisms in very different settings. This is a protocol to model the community-level size spectrum in wadable streams. The protocol consists of three main steps. First, collect quantitative benthic fish and invertebrate samples that can be used to estimate local densities. Second, standardize the fish and invertebrate data by converting all individuals to ataxic units (i.e., individuals identified by size, irrespective of taxonomic identity), and summing individuals within log₂ size bins. Third, use linear regression to model the relationship between ataxic M and D estimates. Detailed instructions are provided herein to complete each of these steps, including custom software to facilitate D estimation and size spectra modeling.

Introduction

Body size scaling relationships, such as the positive association between body mass and metabolic rate, are well-known at the individual organism level and are now being studied at higher levels of organization^1,2,3. These allometric relationships are most often power-law functions of the form Y = aM^b, where Y is the variable of interest (e.g., metabolism, abundance, or home range size), M is the body mass of a single or average individual, b is a scaling coefficient, and a is a constant. For statistical convenience, Y and M data are often log-transformed prior to analysis then modeled with linear equations of the form log (Y) = log (a) + b log (M), where b and log (a) become the linear model slope and intercept, respectively.

The size spectrum is a type of allometric relationship that predicts density (D, the number of individuals per unit area) or biomass (B, the summed mass of individuals per unit area) as a function of M (See Section 4 for additional information on the use of ‘normalized’ D or B estimates.) Like other scaling relationships between M and D or between M and B, the size spectrum plays a central role in basic and applied ecology. At the population-level, biologists often interpret negative D  M relationships as evidence of density-dependent survival or as models of ecosystem carrying capacity (i.e., the ‘self-thinning rule’)^4,5. At the community-level, B  M relationships can be used to study system-level effects of anthropogenic perturbations, such as size-selective fishing^6,7. Allometric scaling of D and B with M are also central to recent efforts to unite population, community, and ecosystem ecology^2,8,9.

One particularly important characteristic of the size spectrum is the fact that it is entirely ataxic^9,10. This point is easy to miss when comparing scatterplots of D  M or B M data but the distinction between taxic and ataxic models is a critical one. In taxic models, a single M value is used to represent the average body mass of every individual of a given species or taxa¹¹. In ataxic models, all individuals within a data set are partitioned among a series of body size intervals or M bins, regardless of their taxonomic identity¹². The latter, ataxic approach is advantageous in aquatic ecosystems where many taxa exhibit indeterminate growth and experience one or more ontogenetic shifts in feeding behavior; in these instances, a single species-level M average will obscure the fact that a species can fill different functional roles throughout its life history^9,13,14.

Here, we present a complete protocol to quantify the size spectrum within wadable streams and rivers. The protocol begins with field sampling methods to collect the necessary fish and benthic macroinvertebrate data. Fish will be collected through a ‘three-pass depletion’ sampling process. Abundance will then be estimated from the depletion data with the Zippin method¹⁵. In depletion sampling, individual fishes within a closed study reach (i.e., individuals can neither enter nor leave the enclosed reach) are removed from the reach through three successive samples. Thus, the number of remaining fishes will be progressively depleted. From this depletion trend, total abundance within the study reach can be estimated then converted to D (in fish per m²), using the known surface area of the study reach. Benthic macroinvertebrates will be collected with standard fixed-area samplers, then identified and measured in the laboratory.

Next, the combined fish and macroinvertebrate data will be partitioned among size bins. Traditionally, the octave or log₂ scale (i.e., doubling intervals) has been used to set size bin boundaries¹⁶. Once a list of size bins has been established, partitioning of individual benthic macroinvertebrates among their respective size bins is straightforward because invertebrates are directly enumerated as numbers of individuals per unit area. However, estimating fish abundances within size bins is more abstract because these estimates are inferred from the depletion data. Detailed instructions are therefore provided to estimate fish abundance within size bins, irrespective of taxonomic identity, from depletion sample data.

Finally, linear regression will be used to model the size spectrum. This protocol is fully compatible with the original, general method of Kerr and Dickie¹⁶ and identical to the methods used by McGarvey and Kirk, 2018¹⁷ in a study of fish and invertebrate size spectra in West Virginia streams. By using this protocol, investigators can insure that their results are directly comparable with other studies that build upon Kerr and Dickie¹⁶, thereby accelerating a broad and robust understanding of body size scaling relationships in freshwater ecosystems and the mechanisms that drive them.

Protocol

All methods described here have been approved by the Institutional Animal Care and Use Committee (IACUC) of Virginia Commonwealth University.

1. Collection and processing of fish samples

1. Isolating fishes within the study reach to create a closed fish assemblage
   1. Identify the upstream and downstream (direction is relative to a surveyor facing ‘upstream’ and against the water current) ends of the study reach then mark the ends with removable flagging tape.
      NOTE: The total length of the study reach is arbitrary but should be long enough to encompass a representative selection of the different habitat types (e.g., riffles, runs, pools, undercut banks) present within the entire stream. In most cases, a 100—200 m study reach will be adequate.
   2. Measure the width of the wetted stream channel at 5—10 transects, distributed evenly along the length of the study reach. Estimate the total surface area of the study reach as the average wetted channel width multiplied by the total length of the reach.
   3. Secure block nets (i.e., knotless seines with floats on the top line and weights on the bottom line) across the stream channel at the upstream and downstream ends of the study reach. Use medium to coarse mesh nets (6.3—9.5 mm or ¼—3/8 inch mesh) to minimize accumulation of debris and clogging. Do not use large (>12.7 mm) mesh nets as small fishes will pass through.
      NOTE: Prior to sampling, prepare a rigging kit that includes: (i) 8 long (≥15 m length) pieces of polypropylene rope (9.5 mm or 3/8 inch diameter); and (ii) 8 cam-action tie-down straps. Do not use ratchet-action straps as these can snap and cause serious injury when their load is released.
      1. At the upstream end of the study reach, locate a tree, root, large rock, or other solid object that can be used to anchor a net on each side of the stream. The availability of suitable anchor points on each side of the stream will likely affect the position of the upstream boundary.
      2. Select one piece of polypropylene rope and create a loop at each end using a bowline knot. Use only a bowline knot, as other knots may become permanently sealed when exposed to moisture and high tension. For instructions on tying a bowline knot, see Figure 1.
      3. Wrap the rope around the tree/root/rock and feed the loop at one end through the loop at the other end to create an anchor point (Figure 2). Shorten or lengthen the rope anchor by adding or removing wraps around the tree/root/rock.
      4. Repeat steps 1.1.3.1—1.1.3.3 to establish a second anchor point on the opposite side of the stream.
      5. Create a loop in the lines at each of the four corners of the block net using a bowline knot. Use only a bowline knot (Figure 1).
      6. Connect both sides of the top line of the block net (the line with floats) to the anchor points using cam-action tie-down straps. Insert the hooks at either end of the tie-down strap into the loops at the corners of the block net and the anchor points (Figure 2). Pull the free tether of the tie-down strap through the cam buckle to tighten each point of contact.
         NOTE: To release tension on the anchors (for adjusting the block net set-up or to remove the block net when sampling is complete), depress the cam button on each of the tie-down straps.
      7. Secure the bottom line of the block net (the line with weights) by pinning them to the stream bank with tent stakes.
      8. Establish a seal with the bottom of the stream using large rocks to pin the block net down. Place rocks on the side of the net facing upstream. Be sure that the top of the net remains above water level (Figure 2). Adjust the height(s) of the anchor point(s) as needed.
      9. Set a second block net by repeating steps 1.1.3.1—1.1.3.8 at the downstream end of the study reach.
2. Perform the first of 3 fish sampling depletion passes within the enclosed study reach. This protocol assumes that a backpack electrofisher is available and all surveying crew personnel are properly trained to use it. Other methods can potentially be used but may not be as effective in collecting representative fish samples.
   NOTE: In small streams, 4—5 people is an ideal crew size: one to operate the electrofisher, two to net stunned fishes, and one or two to carry holding buckets and shuttle captured fishes. Also, backpack electrofishing can cause significant injury, both to stream fishes and humans¹⁸. It is therefore critical to exercise caution and to receive proper training.
   1. Beginning at the downstream end of the enclosed study reach, turn the backpack electrofisher on and move in the upstream direction. Progress slowly, moving side-to-side throughout the study reach to ensure all instream habitats are sampled. The first depletion pass is complete when the upstream net is reached.
   2. Let supporting crew members follow the leader (who is operating the electrofisher), collecting stunned fishes with dip nets as they are spotted and transferring them to temporary buckets, then to aerated holding tubs. Use small battery powered ‘bait bucket’ pumps with aeration stones to ensure that captured fishes remain healthy.
      1. Pay particular attention to very small, young-of-year fishes as they are difficult to spot and capture. When capture of the smallest fishes is highly inefficient, results may be biased. In this event, it may be necessary to remove the smallest log₂ size classes from the fish data, prior to estimating densities within log₂ size bins (see Step 3.2.2).
         NOTE: Success in netting stunned fishes will vary with a number of biological and environmental conditions. For instance, turbid water in which visibility is low will constrain the ability to effectively locate and capture fishes; if turbidity is too high, sampling should be re-scheduled, or an alternate sampling site should be selected.
3. Processing fishes collected in the first depletion pass
   1. Determine whether anesthesia will be needed. Live fishes are often difficult to handle, and sedation may be necessary to minimize stress and injury to fish specimens. If anesthesia is used, two options are widely (as of April 2019) available: Tricaine-S (tricaine methanesulfonate, MS-222) and carbon dioxide (baking soda).
      NOTE: Tricaine-S entails a 21-day holding period before exposed fish can safely be consumed^19,20, but it is currently (as of April 2019) the only fish sedative approved by the U.S. Food and Drug Administration.
   2. When using sedatives, carefully follow all instructions provided with the anesthetic product. In all cases, mix the anesthetic compound in an aerated water bath. Submerge collected fishes in the bath until sedation is observed. Once sedated, process the fishes as quickly as possible, as prolonged exposure to sedatives may cause death.
   3. Use small dip nets to retrieve sampled fishes from the holding tank (with or without sedation), individually or in small batches, for identification. Place the specimens in white plastic or enamel trays and use forceps and magnifying glasses for examination. Use local or regional identification keys (e.g., “The Fishes of Ohio”)²¹ to aid in identification.
   4. Measure total length (from tip of snout to end of caudal fin) for each specimen then weigh on a field balance. If using an electronic balance, select one with 0.1 or 0.01 g precision. Keep a transparent plastic box on hand to use, as necessary, as a wind and rain baffle (it must be large enough to cover the balance and specimens being weighed).
   5. Record all information (species identity, total length, and weight) on waterproof data sheets. A printable example of a fish data sheet is provided in Supplementary File 1.
   6. Once processed, return the fishes to a separate aerated holding/recovery bin. When all fishes have been processed, release them downstream of the downstream block net.
      NOTE: If you accidentally release them into your enclosed study reach, you will ruin your sample! If anesthesia was used, wait to release until all fishes have recovered and regained equilibrium.
4. Performing the second and third depletion passes
   NOTE: If a strong depletion trend is not induced in the first three passes (i.e., if the number of sampled fishes has not noticeably declined by the third pass), additional passes may be needed to accurately estimate fish abundance²². Time permitting, it is often a good idea to proactively conduct four or five successive depletion passes.
   1. Check that the upstream and downstream block nets are still secure. If significant debris has been collected in either block net, remove it by hand picking.
   2. Collect the remaining depletion pass samples by repeating steps 1.2–1.3. Ensure that sampling effort remains consistent among all three passes. Use the same pace of movement (timing the process is recommended) and same crew members to resurvey the sampling reach.
5. When finished, disassemble the block nets and remove all anchor materials.

2. Collection and processing of benthic macroinvertebrate samples

1. Select benthic macroinvertebrate sample sites within the boundaries of the fish sampling reach that are representative of the major types of physical habitats (e.g., riffles or runs) observed in the study reach.
2. Using a fixed-area sampler, collect the first benthic macroinvertebrate sample. In shallow streams with extensive gravel-to-pebble size material, the Surber sampler and Hess sampler are the most commonly used devices but any fixed-area sampler can be used. When sampling other types of habitats where these devices do not work, consult Merritt et al.²³ and Hauer and Resh²⁴.
   1. Place the sampling device firmly against the stream bottom with the sample collection net oriented downstream; move large cobbles as necessary to establish a firm seal with the substrate.
   2. Use a wire or plastic brush to vigorously scrub the substrate within the sampling area for a period of 2 min, allowing dislodged benthic macroinvertebrates to drift into the sample net.
   3. Transfer the sample contents from the net to a plastic jar and cover with 70% isopropyl alcohol for preservation. Label the jar and store it in a safe location for transfer to the lab.
3. Collect and preserve additional benthic macroinvertebrate samples, repeating step 2.2.
   NOTE: The number of macroinvertebrate samples that should be collected is variable and somewhat arbitrary. Ideally, 5—10 replicate samples should be collected and individually preserved. At a minimum, 3 replicate samples should be collected.
4. Return all collected samples to the lab for processing.
   NOTE: Isopropyl alcohol is a flammable liquid and if preserved samples will be shipped via ground or air carrier, it will be necessary to first complete and satisfy all pertinent hazardous goods/dangerous goods training, packing, and shipping requirements.
5. In the lab, sort and identify preserved benthic macroinvertebrate samples.
   1. Separate thespecimens from fine sediment by carefully pouring sample contents into a fine-mesh sieve (e.g., 125 or 250 μm) and rinsing.
   2. Transfer rinsed contents to a white plastic or enamel tray, cover with a small volume of water, and manually pick macroinvertebrates from the remaining residue with fine point forceps. Place extracted macroinvertebrates in a small container of 70% isopropyl alcohol.
      NOTE: If a large amount of coarse plant or mineral residue is mixed with the sample contents, making it difficult to see macroinvertebrates in the tray, it may be necessary to process the remaining sample contents by first subdividing the material and working with several smaller quantities.
   3. Using a dissecting stereo microscope with an ocular micrometer installed in one of the eyepieces, identify specimens to the lowest practical taxonomic level. In most instances, this will be family or genus level.
      NOTE: Processing and identifying the complete contents of a single invertebrate sample will often require 2—5 h or longer. Budget sufficient time and be sure that a suitable library of taxonomic keys^25,26,27,28 is available to assist in identification.
   4. Use the ocular micrometer in the microscope eyepiece to measure the complete body length of each specimen. If body length measurement is not possible (e.g., damaged or missing abdomen), measurement of the head capsule width may suffice.
   5. Estimate individual dry mass (M) for each specimen using the body length or head capsule width measurements and taxon-specific body length vs. M or head width vs. M regression equations from published sources^29,30. For example, the empirical body length (mm) vs. M (mg) equation reported in Benke et al.²⁹ for the alderfly Sialis sp. (Megaloptera, Sialidae) is M = 0.0031 × total length^2.801. Therefore, estimated M for a Sialis sp. specimen with a total length of 15 mm is 6.104 mg.
      NOTE: If a published length vs. M equation is not available for a particular taxon, substitute an appropriate equation at a higher level of taxonomic resolution (e.g., substituting the appropriate family level equation when the genus level equation is not available) or from a closely related taxon with a similar body shape.

3. Estimation of fish and benthic macroinvertebrate densities within log₂ size bins

An animation demonstrating how to format the fish and invertebrate data for use in size spectra analysis is available at http://bit.ly/SizeSpectraDensities.

1. Establish a series of log₂ size bins that will encompass all invertebrate and fish specimens, ranging from the smallest benthic macroinvertebrate to the largest fish. Ensure that all size estimates are in units of mg dry mass.
   NOTE: For consistency, we recommend the size bins used by McGarvey and Kirk¹⁷. These size bins range from 0.0001 to 214,748.3648 mg. A spreadsheet with the lower and upper limits for each of these 31 log₂ size bins is provided in Supplementary File 2.
2. Estimate fish abundance within each of the corresponding size bins.
   1. First convert all individual fish weights from g wet mass (recorded on the field data sheets) to mg dry mass. The wet-to-dry mass conversion factor of Waters³¹ (1 g wet mass = 0.2 g dry mass) can be used after converting from g to mg.
   2. Sum the total number of individual fishes that were captured within each of the respective size bins (irrespective of species identity) during the first, second, and third depletion samples. An example is shown in Supplementary File 2.
      NOTE: Biased under-sampling of very small fishes is common in steam fish sampling and will be evident when individuals are summed within log₂ size bins; summed abundances of the smallest fishes will be conspicuously lower than in larger bins (e.g., 5 vs. 100 individuals in adjacent size bins). Remove log₂ size bins that are clearly biased prior to size spectra analysis (see Step 4).
   3. Use the Zippin maximum likelihood equation^15,32 to estimate total fish abundance (n) within the smallest fish size bin.
      1. Begin by calculating an intermediate X statistic as
         
         where i denotes the i^th sampling pass (i = 1, 2, 3, etc.), k represents the total number of passes (k = 3, unless additional passes were surveyed), and C_i is the total number of fishes captured during the i^th pass.
      2. Calculate the maximum-likelihood estimate of n by iteratively substituting decreasing n values in Equation 2 until
         
         where T is the total number of individuals captured during k passes and all remaining variables are as defined above in Equation 1.
      3. If zero counts are observed in the first, second, or third depletion sample, estimate n as the sum of individuals that were capture among the three depletion samples. A worked example of Equations 1 and 2 is shown in Supplementary File 2.
         NOTE: Several software applications can be used to calculate Zippin abundance estimates from depletion samples, such as the ‘removal’ function in R package ‘FSA’ (Fisheries Stock Assessment)³³. However, it is more instructive to manually solve Equations 1 and 2 in a spreadsheet. Detailed instructions are provided in Lockwood and Schneider³⁴ and Supplementary File 2.
   4. Repeat step 3.2.3 for each of the remaining fish size bins.
   5. Convert the n estimate for each size bin containing fish to a per 1 m²D estimate by dividing n by the total surface area estimate of the surveyed reach from step 1.1.2 above. For instance, if the Zippin n estimate is 70 fish and the surface area of the surveyed reach is 1,200 m², then D = 0.058 fish/m².
3. Estimate benthic macroinvertebrate abundance within log₂ size bins by pooling results from each of the field samples (i.e., combine results from the replicate samples into a single list of individual specimens), then summing the total number of individuals within each size bin.
   NOTE: If the length-mass equations used in step 2.5.5 produce individual weight estimates in units of mg dry mass, no additional unit conversion is necessary for benthic macroinvertebrates.
4. Estimate benthic macroinvertebrate D within each size bin as
   
   For example, if 6 benthic macroinvertebrate samples were collected with a standard Hess device (surface area = 0.086 m²) and a total of 110 individuals were counted within a given size bin, the D estimate for that size bin is 213 individuals/m².
5. Combine D results for fishes and benthic macroinvertebrates into a single table of D estimates per log₂ size bin. If fish and macroinvertebrates occur in the same size bin (a rare event that may occur for the largest invertebrates and smallest fishes), sum their respective D estimates to obtain a total D estimate for that size bin.
6. Delete any ‘empty’ log₂ size bins (i.e., size bins with D values of zero), as empty bins will bias the linear regression models that are used to estimate size spectra parameters^35,36.

4. Modeling the benthic macroinvertebrate and fish size spectrum

1. Estimate the average dry mass for each log₂ size bin (Ḿ) using one of the following values: (i) the minimum value (lower boundary) for each size bin; (ii) the maximum value (upper boundary); (iii) the arithmetic mean (of the minimum and maximum); or (iv) the geometric mean (of the minimum and maximum)³⁵.
   NOTE: In the examples shown below, Ḿ was estimated as the arithmetic mean of each log₂ size bin (see Supplementary File 2).
2. ‘Normalize’ the D estimate for each log₂ size bin by dividing it by its respective width (i.e., difference between the upper and lower boundary)^16,35. This will prevent the non-uniform log₂ size intervals from creating bias in the linear regression models that are used to estimate size spectra parameters^35,37,38.
3. Log₁₀ transform all Ḿ and Ḏ data to convert the curvilinear D  M relationship to a linear relationship. Then use ordinary least squares regression with the log₁₀(Ḿ) and log₁₀(Ḏ) data to model the size spectrum as
   
   where log₁₀(a) is the intercept and b is the slope of the linear size spectrum model.

Results

Exemplar results, including original field data, are presented for Slaunch Fork, West Virginia, a small stream in southern West Virginia. Additional size spectra model results are also presented for two other streams in the same region: Camp Creek and Cabin Creek, West Virginia. These are the three study sites included in McGarvey and Kirk¹⁷, but data presented here are from new samples collected in May 2015. A fully worked, manual example of the size spectra modeling process is included for the S...

Discussion

This ataxic size spectra protocol can be used to quantify and model size structure within communities of stream fishes and invertebrates. Previous size spectra studies in stream ecosystems have ranged from basic descriptive research^39,40 to comparisons along a longitudinal river profile⁴¹ and among distinct biogeographic regions⁴². Seasonal comparisons have been performed^43,

Materials

Name	Company	Catalog Number	Comments
Chest waders	Multiple options	n/a	Personal protective equipment for use during electrofishing. Do NOT use 'breatheable' waders as electrical current will pass through them.
Rubber lineman's gloves	Multiple options	n/a	Personal protective equipment for use during electrofishing.
Dip nets with fiberglass poles	Multiple options	n/a	Used to capture stunned fishes during electrofishing.
Backpack electrofishing unit	Smith-Root; Halltech; Midwest Lake Management; Aqua Shock Solutions	www.smith-root.com; www.halltechaquatic.com; https://midwestlake.com; https://aquashocksolutions.com/	Backpack electrofishers are currently manufactured and distributed by four independent companies in North America. Prices and warranty/technical support are the most important factors in choosing a vendor.
Block nets/seines (×2)	Duluth Nets	https://duluthfishnets.com/	Necessary length will depend on stream width. 3/8 inch mesh is recommended.
Cam-action utility straps with 1 inch nylon webbing (×4)	Multiple options	n/a	Used to secure/anchor block nets. Available at auto supply, hardware, and department stores.
Large tent stakes (×4)	Multiple options	n/a	Used to secure/anchor block nets. Available at camping and department stores.
5 gallon plastic buckets (×5)	Multiple options	n/a	Used to hold and transport fish during electrofishing. Available at hardware and paint supply stores.
10-20 gallon totes (×3)	Multiple options	n/a	Used as livewells, sedation tanks, and recovery bins for captured fishes. Available at hardware and department stores.
Battery powered 'bait bucket' aeration pumps	Cabelas	IK-019008	Used to aerate fish holding bins during field processing.
Fish anesthesia (Tricaine-S)	Syndel	www.syndel.com	Used to sedate fishes for field processing. Tricaine-S is regulated by the U.S. Food and Drug Administration.
Folding camp table and chairs	Cabelas	IK-518976; IK-552777	Used to process fish samples.
Pop-up canopy	Multiple options	n/a	Used as necessary for sun and rain protection.
Fish measuring board	Wildco	3-118-E40	Used to measure fish lengths.
Battery powered field scale with weighing dish	Multiple options	n/a	Used to weigh fishes. Must weigh be accurate to 0.1 or 0.01 grams.
Clear plastic wind/rain baffle	Multiple options	n/a	Used to shield scale in rainy or windy conditions. Must be large enough to cover the scale and a weighing dish.
White plastic or enamel examination trays	Multiple options	n/a	Trays are essential for examining fishes in the field.
Stainless steel forceps	Multiple options	n/a	Forceps are helpful when examining small fishes and in transfering invertebrates to specimen jars.
Hand magnifiers	Multiple options	n/a	Magnification is often helpful when identifying fish specimens in the field.
Fish identification keys	n/a	n/a	Laminated keys that are custom prepared for specific locations are most effective.
Datasheets printed on waterproof paper	Rite in the Rain	n/a	Waterproof paper is essential when working with aquatic specimens.
Retractable fiberglass field tapes	Lufkin	n/a	Used to measure stream channel dimensions.
Surber sampler or Hess sampler	Wildco	3-12-D56; 3-16-C52	Either of these fixed-area benthic samplers will work well in shallow streams with gravel or pebble substrate.
70% ethanol or isopropyl alcohol	Multiple options	n/a	Used as invertebrate preservative.
Widemouth invertebrate specimen jars (20-32 oz.)	U.S. Plastic Corp.	67712	Any widemouth plastic jars will work but these particular jars are durable and inexpensive.