A subscription to JoVE is required to view this content. Sign in or start your free trial.
Abstract
Biochemistry
Emerging research has revealed that the cellular coenzyme A (CoA) supply can become limiting with a detrimental impact on growth, metabolism and survival. Measurement of cellular CoA is a challenge due to its relatively low abundance and the dynamic conversion of free CoA to CoA thioesters that, in turn, participate in numerous metabolic reactions. A method is described that navigates through potential pitfalls during sample preparation to yield an assay with a broad linear range of detection that is suitable for use in many biomedical laboratories.
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved