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Abstract

Developmental Biology

我的老鼠怀孕了吗?高频超声波评估

Published: March 18th, 2021

DOI:

10.3791/61893

1Department of Pediatrics, New York University Grossman School of Medicine, 2Department of Anesthesiology, New York University Grossman School of Medicine, 3Department of Cell Biology, New York University Grossman School of Medicine

Abstract

小鼠是许多人类疾病和生物过程的首选哺乳动物动物模型。发育生物学通常需要分阶段怀孕的小鼠来确定不同时间点的进化过程。此外,模型小鼠的最佳和高效繁殖需要对定时怀孕进行评估。最常见的情况是,小鼠在一夜之间交用,阴道塞的存在被确定:然而,这种技术的正预测值是次优的,人们需要等待知道老鼠是否真的怀孕了。高分辨率超声波生物显微镜是一种有效有效的成像工具:1) 小鼠是否怀孕:2) 鼠标达到的妊娠阶段:和 3) 是否有宫内损失。除了胚胎和胎儿外,研究者还必须识别腹腔中的常见神器,以免误以为这些是子宫。本文提供了成像协议以及说明性示例。

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