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Abstract
Biochemistry
ERRATUM NOTICE
Important: There has been an erratum issued for this article. Read more …This protocol describes a method to obtain subcellular protein fractions from mammalian cells using a combination of detergents, mechanical lysis, and isopycnic density gradient centrifugation. The major advantage of this procedure is that it does not rely on the sole use of solubilizing detergents to obtain subcellular fractions. This makes it possible to separate the plasma membrane from other membrane-bound organelles of the cell. This procedure will facilitate the determination of protein localization in cells with a reproducible, scalable, and selective method. This method has been successfully used to isolate cytosolic, nuclear, mitochondrial, and plasma membrane proteins from the human monocyte cell line, U937. Although optimized for this cell line, this procedure may serve as a suitable starting point for the subcellular fractionation of other cell lines. Potential pitfalls of the procedure and how to avoid them are discussed as are alterations that may need to be considered for other cell lines.
Erratum
Erratum: Cell Fractionation of U937 Cells by Isopycnic Density Gradient PurificationAn erratum was issued for: Cell Fractionation of U937 Cells by Isopycnic Density Gradient Purification. The Authors section was updated.
The Authors section was updated from:
William McCaig1
Timothy LaRocca1
1Department of Basic and Clinical Sciences, Albany College of Pharmacy and Health Sciences
to:
William D. McCaig1
Matthew A. Deragon1
Phillip V. Truong1
Angeleigh R. Knapp1
Keven J. Hughes1
Timothy J. LaRocca1
1Department of Basic and Clinical Sciences, Albany College of Pharmacy and Health Sciences
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