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Abstract
Biology
ERRATUM NOTICE
Important: There has been an erratum issued for this article. Read more …福斯特共振能量转移(FRET)是从激发的供体到受体分子的能量的无辐射转移,取决于分子的距离和方向以及供体发射和受体吸收光谱之间的重叠程度。FRET允许研究活细胞中蛋白质随时间变化和不同亚细胞区室中的相互作用。文献中已经描述了使用显微镜测量FRET的不同基于强度的算法。在这里,提供了一种协议和算法,用于基于测量受体的敏化发射和供体分子的淬灭来量化FRET效率。活细胞中比例FRET的定量不仅需要确定荧光蛋白的串扰(光谱溢出或渗漏),还需要确定显微镜装置的检测效率。此处提供的协议详细说明了如何评估这些关键参数。
Erratum
Erratum: Assessing Protein Interactions in Live-Cells with FRET-Sensitized EmissionAn erratum was issued for: Assessing Protein Interactions in Live-Cells with FRET-Sensitized Emission. The Authors section was updated from:
György Vámosi1
Sarah Miller2
Molika Sinha2
Gabor Mocsár1
Malte Renz2
1Department of Biophysics and Cell Biology, Faculty of Medicine, University of Debrecen
2Gynecologic Oncology Division, Stanford University School of Medicine
to:
György Vámosi1
Sarah Miller2
Molika Sinha2
Maria Kristha Fernandez2
Gabor Mocsár1
Malte Renz2
1Department of Biophysics and Cell Biology, Faculty of Medicine, University of Debrecen
2Gynecologic Oncology Division, Stanford University School of Medicine
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