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	<li>Proikas-Cezanne, T., Takacs, Z., D&#246;nnes, P., Kohlbacher, O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=WIPI+proteins:+Essential+PtdIns3P+effectors+at+the+nascent+autophagosome.">WIPI proteins: Essential PtdIns3P effectors at the nascent autophagosome.</a> Journal of Cell Science. 128 (2), 207-217 (2015).</li><li>Proikas-Cezanne, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=WIPI-1&#945;+(WIPI49),+a+member+of+the+novel+7-bladed+WIPI+protein+family,+is+aberrantly+expressed+in+human+cancer+and+is+linked+to+starvation-induced+autophagy.">WIPI-1&#945; (WIPI49), a member of the novel 7-bladed WIPI protein family, is aberrantly expressed in human cancer and is linked to starvation-induced autophagy.</a> Oncogene. 23 (58), 9314-9325 (2004).</li><li>Bakula, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=WIPI3+and+WIPI4+&#946;-propellers+are+scaffolds+for+LKB1-AMPK-TSC+signalling+circuits+in+the+control+of+autophagy.">WIPI3 and WIPI4 &#946;-propellers are scaffolds for LKB1-AMPK-TSC signalling circuits in the control of autophagy.</a> Nature Communications. 8 (1), 15637 (2017).</li><li>Hayflick, S. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=&#946;-Propeller+protein-associated+neurodegeneration:+A+new+X-linked+dominant+disorder+with+brain+iron+accumulation.">&#946;-Propeller protein-associated neurodegeneration: A new X-linked dominant disorder with brain iron accumulation.</a> Brain. 36, 1708-1717 (2013).</li><li>Wilson, J. A. -. O., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Consensus+clinical+management+guideline+for+beta-propeller+protein-associated+neurodegeneration.">Consensus clinical management guideline for beta-propeller protein-associated neurodegeneration.</a> Developmental Medicine and Child Neurology. 63 (12), 1402-1409 (2021).</li><li>Gao, G., Li, J., Zhang, Y., Chang, Y. Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cellular+iron+metabolism+and+regulation.">Cellular iron metabolism and regulation.</a> Advances in Experimental Medicine and Biology. 1173, 21-32 (2019).</li><li>Mancias, J. D., Wang, X., Gygi, S. P., Harper, J. W., Kimmelman, A. 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<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>Cell lines</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Primary skin-derived human fibroblasts&#160;</td>
			<td>Skin biopsy, healthy human donor, Biobank University Clinic T&#252;bingen, Ethical approvement 389/2019BO2</td>
			<td>F-CO-60, passage 9</td>
			<td></td>
		</tr>
		<tr>
			<td>Cell culture treatments</td>
			<td></td>
			<td></td>
			<td>Final concentration (compound)</td>
		</tr>
		<tr>
			<td>Control medium</td>
			<td>DMEM 10%FCS</td>
			<td></td>
			<td>(DMSO only)</td>
		</tr>
		<tr>
			<td>Control medium + bafilomycinA1</td>
			<td>DMEM 10%FCS</td>
			<td></td>
			<td>100 nM bafilomycin A1</td>
		</tr>
		<tr>
			<td>Control medium + deferasiox (DFX)</td>
			<td>DMEM 10%FCS</td>
			<td></td>
			<td>30 &#181;M DFX</td>
		</tr>
		<tr>
			<td>Control medium + DFX + bafilomycinA1</td>
			<td>DMEM 10%FCS</td>
			<td></td>
			<td>30 &#181;M DFX, 100 nM bafilomycin A1</td>
		</tr>
		<tr>
			<td>Control medium + ferric ammonium citrate (FAC)</td>
			<td>DMEM 10%FCS</td>
			<td></td>
			<td>0.05 mg/mL FAC</td>
		</tr>
		<tr>
			<td>Control medium + FAC + bafilomycinA1</td>
			<td>DMEM 10%FCS</td>
			<td></td>
			<td>0.05 mg/mL FAC, 100 nM bafilomycin A1</td>
		</tr>
		<tr>
			<td>Material</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Albumin [BSA] Fraction V</td>
			<td>AppliChem</td>
			<td>A1391</td>
			<td></td>
		</tr>
		<tr>
			<td>Alexa 488 goat a-rabbit</td>
			<td>Life Technologies</td>
			<td>A-11008</td>
			<td></td>
		</tr>
		<tr>
			<td>Alexa 546 goat a-mouse</td>
			<td>Life Technologies</td>
			<td>A-11003</td>
			<td></td>
		</tr>
		<tr>
			<td>Bafilomycin A1</td>
			<td>Sigma Aldrich</td>
			<td>196000</td>
			<td></td>
		</tr>
		<tr>
			<td>BioLite Cell Culture treated 10cm dishes</td>
			<td>Thermo Scientific</td>
			<td>130182</td>
			<td></td>
		</tr>
		<tr>
			<td>DAPI</td>
			<td>AppliChem</td>
			<td>A4099</td>
			<td></td>
		</tr>
		<tr>
			<td>Deferasiox (ICL-670)</td>
			<td>Selleckchem</td>
			<td>S1712</td>
			<td></td>
		</tr>
		<tr>
			<td>DMEM Glutamax</td>
			<td>Gibco</td>
			<td>31966</td>
			<td></td>
		</tr>
		<tr>
			<td>DMSO&#160;</td>
			<td>AppliChem</td>
			<td>A3672</td>
			<td></td>
		</tr>
		<tr>
			<td>DPBS no calcium nor magnesium</td>
			<td>Gibco</td>
			<td>14190094</td>
			<td></td>
		</tr>
		<tr>
			<td>Fetal bovine serum (FCS)</td>
			<td>Gibco</td>
			<td>10437-028</td>
			<td></td>
		</tr>
		<tr>
			<td>Ferric ammonium citrate (FAC)</td>
			<td>Merck</td>
			<td>F5879</td>
			<td></td>
		</tr>
		<tr>
			<td>Anti-FERRITIN (Human Spleen)</td>
			<td>Rockland</td>
			<td>200-401-090-0100</td>
			<td></td>
		</tr>
		<tr>
			<td>LAMP2 (H4B4)</td>
			<td>Santa Cruz</td>
			<td>Sc-18822</td>
			<td></td>
		</tr>
		<tr>
			<td>Paraformaldehyde (PFA)</td>
			<td>Sigma-Aldrich</td>
			<td>441244</td>
			<td></td>
		</tr>
		<tr>
			<td>PBS 10x Dulbecco&#8217;s</td>
			<td>AppliChem</td>
			<td>A0965</td>
			<td></td>
		</tr>
		<tr>
			<td>PenStrep (1,00U/mL penicillin, 100 mg/mL streptomycin)</td>
			<td>Life Technologies</td>
			<td>15140-122</td>
			<td></td>
		</tr>
		<tr>
			<td>Trypsin-EDTA (0.05%) with phenol red</td>
			<td>Gibco</td>
			<td>25300</td>
			<td></td>
		</tr>
		<tr>
			<td>Tween20</td>
			<td>AppliChem</td>
			<td>A4974</td>
			<td></td>
		</tr>
		<tr>
			<td>96 well glass-bottom #0 plates</td>
			<td>Cellvis</td>
			<td>P96-0-N</td>
			<td></td>
		</tr>
		<tr>
			<td>Solution</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>3.7% paraformaldehyde (PFA)</td>
			<td></td>
			<td></td>
			<td>PFA dissolved in PBS, pH 7.5&#160;</td>
		</tr>
		<tr>
			<td>Bafilomycin A1 100 mM stock</td>
			<td></td>
			<td></td>
			<td>Bafilomycin A1 diluted in DMSO</td>
		</tr>
		<tr>
			<td>Ferric ammonium citrate (FAC) 100 mg/mL stock</td>
			<td></td>
			<td></td>
			<td>FAC diluted in autoclaved double distilled water</td>
		</tr>
		<tr>
			<td>PBS/T</td>
			<td></td>
			<td></td>
			<td>PBS, supplemented with 10% Tween20&#160;</td>
		</tr>
		<tr>
			<td>PBS/T + BSA</td>
			<td></td>
			<td></td>
			<td>PBS, supplemented with 10% Tween20, 1% BSA&#160;</td>
		</tr>
		<tr>
			<td>Software</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>CellProfiler 4.2.4</td>
			<td>https://cellprofiler.org/</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>GraphPad Prism</td>
			<td>Dotmatics</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Microsoft Excel Version 16.50</td>
			<td>Microsoft Office Plus 365</td>
			<td></td>
			<td></td>
		</tr>
	</tbody>
</table>

ferritinophagy: assessing the selective degradation of iron by autophagy in human fibroblasts

Watch this Scientific Journal Video about Ferritinophagy: Assessing the Selective Degradation of Iron by Autophagy in Human Fibroblasts at JoVE.com

<meta charset="utf8"></head><body>Ferritinophagy: تقييم التدهور الانتقائي للحديد عن طريق الالتهام الذاتي في الخلايا الليفية البشرية

Ferritinophagy: تقييم التدهور الانتقائي للحديد عن طريق الالتهام الذاتي في الخلايا الليفية البشرية

Mutations in the autophagy gene WDR45/WIPI4 are the cause of beta-propeller-associated neurodegeneration (BPAN), a subtype of human diseases known as ...

ferritinophagy-assessing-selective-degradation-iron-autophagy-human

Ferritinophagy: تقييم التدهور الانتقائي للحديد عن طريق الالتهام الذاتي في الخلايا الليفية البشرية

Abstract