Abstract
Immunology and Infection
* These authors contributed equally
ERRATUM NOTICE
Important: There has been an erratum issued for this article. Read more …To monitor the progression of infectious diseases, it is useful to assess immunoreactivity against various antigenic determinants, and measure different antibody isotypes because they appear at different stages of the host immune response. With Lyme borreliosis, the pathogenic agent can be one of the multiple members of the Borrelia species. Therefore, correct sample classification requires evaluating the immunoreactivity against different antigens of different Borrelia species. Additionally, anti-pathogen IgG and IgM responses can have different elicitation time courses during disease progression. Here we demonstrate the development of a two-reporter multiplex immunoassay that has utility in identifying Borrelia-specific immune response in human serum samples by simultaneously evaluating both IgG and IgM immunoreactivity against different bacterial antigens in the same reaction well. This dual-reporter approach retains the analytical performance of single-reporter methods while conserving time and resources and reducing sample size requirements. This assay allows essentially double the serological information to be generated from a blood sample in half the time.
Erratum
Erratum: Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological TestAn erratum was issued for: Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological Test. The Authors section was updated to correct an Affiliation. It was updated from:
Julia Häring1
Tanja Michel1
Matthias Becker1
Daniel Junker1
Tatia Tchitchagua2
Olaf Leschnik2
Berit Lange3,4
Stefanie Castell3,4
Gérard Krause3,4
Monika Strengert3
Alex Dulovic1
Nicole Schneiderhan-Marra1
1NMI Natural and Medical Sciences Institute, University of Tübingen
2Department of Neurology, Sächsisches Krankenhaus Rodewisch
3Department of Epidemiology, Helmholtz Centre for Infection Research
4German Centre for Infection Research (DZIF)
to:
Julia Häring1
Tanja Michel1
Matthias Becker1
Daniel Junker1
Tatia Tchitchagua2
Olaf Leschnik2
Berit Lange3,4
Stefanie Castell3,4
Gérard Krause3,4
Monika Strengert3
Alex Dulovic1
Nicole Schneiderhan-Marra1
1NMI Natural and Medical Sciences Institute at the University of Tübingen
2Department of Neurology, Sächsisches Krankenhaus Rodewisch
3Department of Epidemiology, Helmholtz Centre for Infection Research
4German Centre for Infection Research (DZIF)
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