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Abstract

Biology

Mouse Lumbar Vertebra Uniaxial Compression Testing with Embedding of the Loading Surface

Published: December 1st, 2023

DOI:

10.3791/65502

1Department of Biology, Colorado State University-Pueblo, 2Hackensack Meridian School of Medicine, 3Department of Engineering, Colorado State University-Pueblo

Abstract

There is increasing awareness that cortical and cancellous bone differ in regulating and responding to pharmaceutical therapies, hormone therapies, and other treatments for age-related bone loss. Three-point bending is a common method used to assess the influence of a treatment on the mid-diaphysis region of long bones, which is rich in cortical bone. Uniaxial compression testing of mouse vertebrae, though capable of assessing bones rich in cancellous bone, is less commonly performed due to technical challenges. Even less commonly performed is the pairing of three-point bending and compression testing to determine how a treatment may influence a long bone's mid-diaphysis region and a vertebral centrum similarly or differently. Here, we describe two procedures to make compression testing of mouse lumbar vertebrae a less challenging method to perform in parallel with three-point bending: first, a procedure to convert a three-point bending machine into a compression testing machine, and second, an embedding method for preparing a mouse lumbar vertebra loading surface.

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Keywords Mouse Lumbar Vertebra

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