Static Strength Training Method for Type 2 Diabetic Mice

Summary

This protocol provides a simple method of making static training equipment for mice. The device maintains the muscle isometric contraction of the limbs of mice so as to verify the intervention effect of traditional exercise on type 2 diabetes (T2DM) and provides new exercise therapy for the clinical treatment of T2DM.

Abstract

The treatment of type 2 diabetes mellitus (T2DM) is a major difficulty in improving patient health. Exercise is one of the main interventions for T2DM. Static strength training is one of the key forms of traditional sports in China. Research shows that static strength training is an effective clinical method for T2DM intervention, but there is no experimental device suitable for static training in mice. One of the difficulties in moving from clinical to basic research is to design appropriate experimental devices. In order to further study the mechanism of static training intervention in T2DM, a simple method for making a static training device for mice is introduced in this paper. This device has the advantages of simple operation, cheap material, and high feasibility. Previous studies conducted under this protocol have shown that static training can effectively reduce blood glucose levels and improve the mitochondrial function of skeletal muscle cells in T2DM mice. The purpose of introducing this device is to promote research on the mechanism of traditional exercise in the intervention of T2DM and to lay a foundation for the quantitative intervention of exercise.

Introduction

Type 2 diabetes mellitus (T2DM) is a chronic disease characterized by insulin resistance and β-cell dysfunction that is a significant threat to global health¹. Exercise is a crucial intervention in the management of type 2 diabetes. Numerous studies have shown that traditional Chinese exercise methods, such as Tai Chi and Ba Duan Jin, significantly improve blood glucose levels and quality of life for individuals with T2DM^2,3,4,5. To execute these movements, the trainer must maintain a stable body and joint positi....

Protocol

All animal experiments were approved by the Animal Care and Use Committee of the Nanjing University of Chinese Medicine (permission no. 202209A033). Healthy male C57BL/6J mice with SPF grade, 8 weeks of age, and body weight of 20 ± 4 g were selected. The mice were housed in a 12 h light/dark cycle at a temperature of 20-22 °C, and a relative humidity of 45%-50% was maintained. The animals eat and drink freely.

1. Establishment of a mouse model of T2DM

1. Fee.......

Discussion

Static strength training can reduce fat accumulation, aid weight loss, and increase metabolism⁸. In addition, it enhances the expression of PGC-1α and mitochondrial biogenesis in skeletal muscle cells, leading to improved glucose metabolism in mice with type 2 diabetes mellitus and a consequent reduction in blood glucose levels¹¹. To confirm the impact and mechanism of static training on T2DM, appropriate devices need to be developed for performing static training on e.......

Materials

Name	Company	Catalog Number	Comments
Acrylic boards			Transparent acrylic boards with 5mm thickness. The size should be larger than 20cm×20cm
Boxes			Two boxes of the same height (15~20cm)
ELISA KIT		H203-1-2	Nanjing Jiancheng Bioengineering Institute
Hot melt glue gun			Avoid touching the gun head to cause burns
Knives			No special requirement
Metformin tablets		1396309	Sigma
scissors			No special requirement
Sticks			Several wooden sticks with a diameter of 3mm
Streptozotocin		S0130	Sigma
Tape			No special requirement
Transmission Electron Microscope (TEM)		HT7700	HITACHI