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Abstract

Medicine

使用灌注小鼠肝脏模型量化肝葡萄糖生成、尿素生成和脂肪分解

Published: October 6th, 2023

DOI:

10.3791/65596

1Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 2NNF Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, 3Department for Clinical Biochemistry, Copenhagen University Hospital - Bispebjerg and Frederiksberg, 4Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, 5NNF Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen

Abstract

肝脏具有多种功能,包括营养代谢。与其他体 体内 肝脏研究模型相比,分离的灌注肝脏允许研究整个肝脏的肝脏生物学和代谢,具有完整的肝脏结构,不受肝外因素的影响。肝脏灌注最初是为大鼠开发的,但该方法也适用于小鼠。在这里,我们描述了小鼠肝脏 原位 灌注的方案。用含氧的Krebs-Henseleit碳酸氢盐缓冲液通过门静脉对肝脏进行顺行灌注,并从肝上下腔静脉收集输出物,并夹紧肝下腔静脉以关闭回路。使用这种方法,可以用详细的时间分辨率评估测试化合物的直接肝脏效应。肝功能和活力稳定至少3小时,允许在同一实验中加入内部对照。使用该模型的实验可能性很多,可以推断出对肝脏生理学和肝脏疾病的见解。

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