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Abstract

Bioengineering

3D生物打印光可调水凝胶研究成纤维细胞活化

Published: June 30th, 2023

DOI:

10.3791/65639

1Department of Bioengineering, University of Colorado Denver | Anschutz Medical Campus, 2Department of Pediatrics, University of Colorado Anschutz Medical Campus, 3Division of Pulmonary Sciences and Critical Care Medicine, Department of Medicine, University of Colorado Anschutz Medical Campus

Abstract

光可调水凝胶可以响应光照在空间和时间上发生转变。将这些类型的生物材料纳入细胞培养平台并动态触发变化,例如增加微环境硬度,使研究人员能够模拟纤维化疾病进展过程中发生的细胞外基质 (ECM) 变化。本文提出了一种用于3D生物打印能够在明胶支持浴内进行两次连续聚合反应的光电可调水凝胶生物材料的方法。通过调节支持浴的pH值来适应悬浮水凝胶的自由形式可逆嵌入(FRESH)生物打印技术,以促进Michael加成反应。首先,将含有聚乙二醇-α-甲基丙烯酸酯(PEGαMA)的生物墨水与细胞可降解交联剂进行化学计量反应,形成软水凝胶。这些软水凝胶随后暴露在光引发剂和光下,以诱导未反应基团的均聚化并使水凝胶变硬。该协议涵盖水凝胶合成、3D 生物打印、光强化和终点表征,以评估 3D 结构内的成纤维细胞活化。这里介绍的方法使研究人员能够3D生物打印各种经历pH催化聚合反应的材料,并可用于设计组织稳态,疾病和修复的各种模型。

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