Utilizing Thermal Shift Assay to Probe Substrate Binding to Selenoprotein O

Summary

Here, we present the thermal shift assay, a high-throughput, fluorescence-based technique used to investigate the binding of small molecules to proteins of interest.

Abstract

Defining the biological importance of proteins with unknown functions poses a significant obstacle in understanding cellular processes. Although bioinformatic and structural predictions have contributed to the study of unknown proteins, in vitro experimental validations are often hampered by the optimal conditions and cofactors required for biochemical activity. Cofactor binding is not only essential for the activity of some enzymes but may also enhance the thermal stability of the protein. One practical application of this phenomenon lies in utilizing the change in thermal stability, as measured by alterations in the protein's melting temperature, to probe ligand binding.

Thermal shift assay (TSA) can be used to analyze the binding of different ligands to the protein of interest or find a stabilizing condition to perform experiments such as X-ray crystallography. Here we will describe a protocol for TSA utilizing the pseudokinase, Selenoprotein O (SelO), for a simple and high-throughput method for testing metal and nucleotide binding. In contrast to canonical kinases, SelO binds ATP in an inverted orientation to catalyze the transfer of AMP to the hydroxyl side chains of proteins in a posttranslational modification known as protein AMPylation. By leveraging the shift in the melting temperatures, we provide crucial insights into the molecular interactions underlying SelO function.

Introduction

Much of the human proteome remains poorly characterized. The "streetlight effect" described by Dunham and Kustatscher et al. refers to the phenomenon where extensively researched proteins receive more attention, leaving understudied proteins overlooked^1,2. Factors contributing to this effect include the biological importance and disease relevance of certain proteins. Moreover, prior research that offers foundational knowledge, as well as the availability of tools for functional analysis, further stimulates research on highly studied proteins^1,2. Projects such as the Understudied Proteins Initiative, Structural Genomics Consortium, and Enzyme Function Initiative aim to characterize understudied proteins using structural and functional proteomics^2,3,4. A complementary approach to identifying the molecular functions of understudied proteins is to examine the interactions of these proteins with small molecules to gain valuable insights into the regulation and substrate specificity.

The binding of small molecules can increase the thermal stability of a protein⁵. This phenomenon, known as ligand-induced conformation stabilization, often results in an increase in the melting temperature of a protein, providing a measurable indication of ligand binding⁶. The thermal stability of a protein can be measured using Differential Scanning Fluorimetry (DSF), also known as Thermofluor, or Thermal Shift Assay (TSA)^7,8,9,10. In TSA, a protein sample is subjected to increasing temperatures in the presence of an environmentally sensitive dye⁶. As the protein unfolds and denatures, the dye binds to the exposed hydrophobic regions of the protein and emits fluorescence. Extrinsic fluorescent dyes, or environmentally sensitive dyes, lack intrinsic fluorescence and instead fluoresce upon interacting with their target molecule^9,11,12. The most commonly used dye, SYPRO Orange, offers several advantages such as enhanced stability and minimal background fluorescence^8,9,12. Notably, SYPRO Orange is compatible with Real-Time Polymerase Chain Reaction (RT-PCR) systems given its excitation and emission wavelengths around 470 nm and 570 nm, respectively. This unique feature allows for high-throughput, accurate, and sensitive measurements that are compatible with fluorescence detection systems commonly used in RT-PCR systems⁸.

TSA is a versatile tool for investigating protein interactions with potential cofactors or drugs and for identifying stabilizing conditions for crystallography. Some of its advantages over other screening methods are its relative simplicity of setup and high throughput with 96- or 384-well plates^13,14,15. The development of this technique has been transformative for drug discovery studies, offering convenience and enabling the study of diverse additives such as ions, ligands, and drugs^6,16. Moreover, the adaptability of TSA has encouraged scientists to expand its utility, facilitating the measurement of binding affinities alongside the screening assays^17,18. TSA is an effective tool in structural biology studies to identify conditions that promote the stabilization of the protein of interest for crystallization¹⁹. Thus, its flexibility and relative ease have positioned TSA as a cornerstone technique in characterizing proteins. Here, we will use TSA to analyze the binding of metals and nucleotide to the understudied pseudokinase, Selenoprotein O (SelO), as an example.

Kinases are one of the most targeted protein families in drug discovery²⁰. Approximately 10% of human kinases are predicted to be inactive and named pseudokinases because they lack key catalytic residues required for catalysis^21,22. Selenoprotein O (SelO) is an evolutionarily conserved pseudokinase that lacks the conserved aspartate in the catalytic HRD motif²³. In eukaryotes, SelO localizes to the mitochondria and protects cells from oxidative stress^24,25. Structural and biochemical analyses show that SelO transfers adenosine monophosphate (AMP) from ATP to protein substrates in a posttranslational modification known as AMPylation²⁵. Recent studies indicate that enzymes that catalyze AMPylation may bind alternative nucleotides such as UTP in vitro^26,27. Notably, studies have demonstrated that the SelO homolog from Salmonella typhimurium catalyzes protein UMPylation, or the transfer of UMP, in a manganese-dependent manner²⁸. Given these intriguing observations, we test the binding of SelO to ATP and UTP in the presence of magnesium and manganese, serving as a representative example of TSA's applications. The following protocol can be readily adapted to optimize and examine the interactions of other proteins and additives of interest.

Protocol

1. Experimental setup

1. Use a thermal cycler that can detect fluorescence, such as an RT-PCR instrument, to perform the described protocol. If using SYPRO Orange, as described in this protocol, use a scanning mode that allows for excitation around 470 nm and emission detection around 570 nm. Program the thermocycler to hold the sample at 20 °C for 2 min, followed by an increase in temperature of 0.5 °C/1 min up to 95 °C; measure fluorescence intensity every 1 °C.
   NOTE: We recommend an optional step at the end of the cycle to return the sample block to 20 °C (Figure 1).
2. Each reaction contains four components: Buffer, protein of interest, additive, and extrinsic fluorescent dye. Design the experiment to include controls such as no protein, no dye, protein without additives, and additives alone to determine any background fluorescence that may influence the interpretation of the results. If available, include a positive control such as an additive known to bind and stabilize the protein of interest.
3. Use purified protein for the assay. To follow this example, use E. coli SelO, expressed and purified as previously described^25,29. Briefly, express His-sumo tagged SelO (E. coli SelO ppSumo) in Rosetta DE3 cells and purify it using Ni²⁺-NTA affinity. Cleave the His-sumo tag using Ulp protease and purify SelO further by gel filtration chromatography.
4. The fluorescence of SYPRO Orange will depend on its interaction with the protein of interest. As these interactions will vary from protein to protein, optimize the protein and dye concentration for obtaining the maximum signal to noise of fluorescence signal.
   NOTE: SYPRO Orange is not compatible with some proteins and additives such as detergents^11,30. Additional extrinsic dyes listed in ¹² may be screened as necessary.

2. Determination of the optimal dye concentration (Figure 2A)

1. Prepare 20 µL dilutions of 50x, 40x, 30x, 20x, and 10x SYPRO Orange from the stock solution, which is provided at 5,000x in DMSO.
2. Dispense 8 µL of 6.25 µM protein diluted in TSA buffer into six separate wells of a 384-well PCR plate.
   NOTE: In our example, we used the following TSA buffer to perform the dilutions: 10 mM Tris pH 8, 150 mM NaCl, and 1 mM DTT. Samples may be run in triplicate to improve the reliability of outcomes.
3. Add 1 µL of TSA buffer to each well.
   NOTE: This volume of buffer will be replaced with the addition of small molecules after optimization of dye and protein concentration.
4. Add 1 µL of each serial dilution of SYPRO Orange dye solution to each well. Add 1 µL of buffer without dye to the final well for the no dye control.
5. Cover the plate with optically clear adhesive film.
6. Briefly spin down the plate at 1,000 × g for 1 min in a centrifuge equipped with a PCR plate adapter.
7. Place the plate in an RT-PCR machine and start the thermocycling protocol described in step 1.1.

3. Determination of the optimal protein concentration (Figure 2B)

1. Prepare 20 µL of serial dilutions of 25 µM, 12.5 µM, 6.25 µM, 3.125 µM, and 1.56 µM of protein using TSA buffer (10 mM Tris pH 8, 150 mM NaCl, and 1 mM DTT).
2. Dispense 8 µL of serial dilutions of the protein into five separate wells of a 384-well plate.
3. Dispense buffer only in the 6^th well as a no protein control.
4. Add 1 µL of TSA buffer to each well.
   NOTE: This volume of buffer will be replaced with the addition of small molecules after optimization of dye and protein concentration.
5. Add 1 µL of each 50x SYPRO Orange dye solution to each well.
6. Cover the plate with optically clear adhesive film.
7. Briefly spin down the plate at 1,000 × g for 1 min in a centrifuge equipped with a PCR plate adapter.
8. Place the plate in an RT-PCR machine and start the thermocycling protocol described in step 1.1.

4. Setting up a TSA experiment (Figure 3A)

NOTE: After optimization of the protein and SYPRO Orange dye concentrations, the assay can be performed with the addition of the desired small molecules to analyze binding.

1. Define the number of conditions by considering the replicates and adequate controls.
   NOTE: For example, we will test the binding of SelO to eight conditions in triplicate. Including the controls (no additive, no protein, and no dye), we have 11 reactions x 3 (triplicate) = 33 total reactions.
2. Based on the optimal concentration of protein and dye observed for SelO, make sure that each reaction contains 8 µL of 6.25 µM protein, 1 µL of additive, and 1 µL of 50x SYPRO Orange dye for a total of 10 µL reaction volume. Thus, the final concentration used is 5 µM protein and 5x SYPRO Orange dye.
3. Prepare 288 µL of 6.25 µM protein solution using TSA buffer. This volume of protein working solution accounts for 36 reactions (33 reactions with an additional 10% for variations in pipetting).
4. Dispense 8 µL of 6.25 µM protein into separate wells of a 384-well plate. Dispense buffer only for the no protein control.
5. Add 1 µL of small molecules at 10x concentration to achieve final 1x. To follow this example, add 1 µL of 20 mM MgCl₂ to achieve a final concentration of 2 mM. Dispense buffer only for the no additive control.
6. Add 1 µL of each 50x SYPRO Orange dye solution to each well.
7. Cover the plate with optically clear adhesive film.
8. Briefly spin down the plate at 1,000 × g for 1 min in a centrifuge equipped with a PCR plate adapter.
9. Place the plate in an RT-PCR machine and start the thermocycling protocol described in step 1.1.

5. Data analysis

1. Export data from the RT-PCR machine for relative fluorescence units (RFU) with respect to temperature (°C).
2. Use the software of choice to extract and plot data points up to the highest intensity measured for each melting curve. Importantly, confirm that the no protein and no dye controls exhibit low background fluorescence without temperature-dependent increases in fluorescence (Figure 3A,B and Supplemental Table S1). Perform data analysis using freely accessible software such as MoltenProt³¹ or DSFWorld³².
3. Determine the melting temperature by using Boltzmann Sigmoidal Fit for the data. The melting temperature (T_m) is defined as the temperature at which 50% denaturation or half maximal intensity is observed (Figure 3C).
4. A thermal shift in the melting curve may suggest stabilizing or destabilizing additives. To calculate the change of melting temperature (T_m), use the formula: T_m= T_m additive_- T_m buffer. A positive value describes a stabilizing condition or interacting additive; a negative value describes a destabilizing condition (Figure 3D).

Results

Eukaryotic SelO consists of an N-terminal mitochondrial targeting sequence, a kinase-like domain, and a highly conserved selenocysteine at the C-terminus of the protein²³. This mitochondrial-resident enzyme encodes a pseudokinase domain that is conserved from bacteria to humans²³. Structural analysis of the SelO homolog from Pseudomonas syringae revealed amino acid alterations in the active site that facilitate the binding of ATP in an inverted orientation in compa...

Discussion

The Thermal Shift Assay (TSA) serves as an efficient method for screening protein-ligand interactions, including those with cofactors and inhibitors. In this protocol, we used TSA to measure the binding of the pseudokinase SelO to nucleotides and divalent cations. Our findings show that SelO exhibits increased thermal stability in the presence of ATP and Mg²⁺/Mn²⁺. This observation aligns with previous reports indicating that SelO homologs from E. coli, S. cerevisiae, and H. sapiens

Materials

Name	Company	Catalog Number	Comments
Adenosine 5′-triphosphate disodium salt hydrate	Sigma Aldrich	A2383-10G	used for representative results but not required to perfom TSA
Avanti J-15R with microplate carrier assembly	Beckman Coulter	C19416
CFX Opus 384 Real-time PCR system	Bio-Rad	12011452
Hard-Shell 384-Well PCR Plates, thin wall, skirted, clear/white	Bio-Rad	HSP3805
Magnesium Chloride	Sigma Aldrich	M8266-100G	used for representative results but not required to perfom TSA
Manganese (II) chloride tetrahydrate	Sigma Aldrich	M3634-500G	used for representative results but not required to perfom TSA
Microseal 'B' PCR Plate Sealing Film, adhesive, optical	Bio-Rad	MSB1001
SYPRO Orange Protein Gel stain	Sigma Aldrich	S5692-500UL
Uridine 5′-triphosphate trisodium salt hydrate	Sigma Aldrich	U6625-100MG	used for representative results but not required to perfom TSA