Quantification of Global Histone Post Translational Modifications Using Intranuclear Flow Cytometry in Isolated Mouse Brain Microglia

1.9K Views

•

07:10 min

•

September 15th, 2023

DOI :

10.3791/65080-v

September 15th, 2023

•

Morgan Towriss¹, Jennifer Kim², Annie Vogel Ciernia¹

¹Department of Biochemistry and Molecular Biology, Djavad Mowafaghian Centre for Brain Health, University of British Columbia, ²Graduate Program in Neuroscience, Djavad Mowafaghian Centre for Brain Health, University of British Columbia

Transcript

Summary

Explore More Videos

Histone Post translational Modifications

Intranuclear Flow Cytometry

Microglia

Gene Expression Regulation

Neurodevelopmental Conditions

Neuropsychiatric Conditions

Epigenetic Modifications

High throughput Screening

Chromatin Structure Modifications

Acetylation

Transcription Factor Access

Quantitative Analysis

Multiplexing

Genome Sequencing Approaches

Chapters in this video

0:00

Introduction

1:04

Mouse Brain Dissection for Microglia Isolation

2:02

Mouse Brain Immune‐Enriched Fragment Isolation for Histone PTM Quantification

3:37

Mouse Brain Enriched Immune Pellet Plate Staining Method for Histone Quantification

4:48

Flow Cytometry and Data Analysis of Murine Brain Histone Post‐Translational Modifications

Related Videos

11:14

Isolating LacZ-expressing Cells from Mouse Inner Ear Tissues using Flow Cytometry

11.2K Views

10:15

Intracerebroventricular Viral Injection of the Neonatal Mouse Brain for Persistent and Widespread Neuronal Transduction

58.3K Views

08:15

Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes

8.0K Views

12:34

Analysis of Immune Cells in Single Sciatic Nerves and Dorsal Root Ganglion from a Single Mouse Using Flow Cytometry

11.4K Views

09:43

Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue

21.1K Views

14:25

Quantification of Reactive Oxygen Species Using 2′,7′-Dichlorofluorescein Diacetate Probe and Flow-Cytometry in Müller Glial Cells

6.3K Views

01:38

Author Spotlight: Advancing Understanding Through Technological Innovations in Psychoneuroimmunology

596 Views

01:11

Author Spotlight: Enhanced Multiplex Immunofluorescent Microscopy Protocol for Neuroscience Research

240 Views

04:13

Analysis of Immune Cell Diversity Using Histo-Flow Cytometry

50 Views