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University of Veterinary Medicine Hannover

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Methods to Study Lipid Alterations in Neutrophils and the Subsequent Formation of Neutrophil Extracellular Traps

Graham Brogden^*1,2, Ariane Neumann^*1,3, Diab M. Husein¹, Friederike Reuner^1,4, Hassan Y. Naim¹, Maren von Köckritz-Blickwede^1,4

¹Department of Physiological Chemistry, University of Veterinary Medicine Hannover, ²Fish Disease Research Unit, University of Veterinary Medicine, ³Department of Clinical Sciences, Biomedical Center, Lund University, ⁴Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover

Lipids are known to play an important role in cellular functions. Here, we describe a method to determine the lipid composition of neutrophils, with emphasis on the cholesterol level, by using both HPTLC and HPLC to gain a better understanding of the underlying mechanisms of neutrophil extracellular trap formation.

Medicine

A Model to Simulate Clinically Relevant Hypoxia in Humans

Lars Eichhorn¹, Florian Kessler¹, Volker Böhnert², Felix Erdfelder¹, Anja Reckendorf³, Rainer Meyer⁴, Richard K. Ellerkmann¹

¹Department of Anaesthesiology and Intensive Care Medicine, University Hospital of Bonn, ²Institute of Clinical Chemistry and Clinical Pharmacology, University of Bonn, ³Institute for Terrestrial and Aquatic Wildlife Research, University of Veterinary Medicine Hannover, ⁴Institute of Physiology 2, University of Bonn

Hypoxia simulation in humans has usually been performed by inhaling hypoxic gas mixtures. For this study, apneic divers were used to simulate dynamic hypoxia in humans. Additionally, physiological changes in desaturation and re-saturation kinetics were evaluated with non-invasive tools such as Near-Infrared-Spectroscopy (NIRS) and peripheral oxygenation saturation (SpO₂).