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Louis Stokes Cleveland Department of Veterans Affairs Medical Center

7 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Environmentally-controlled Microtensile Testing of Mechanically-adaptive Polymer Nanocomposites for ex vivo Characterization
Allison E. Hess 1, Kelsey A. Potter 1,2, Dustin J. Tyler 1,2, Christian A. Zorman 1,3, Jeffrey R. Capadona 1,2
1Advanced Platform Technology Center, Rehabilitation Research and Development, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, 2Department of Biomedical Engineering, Case Western Reserve University, 3Department of Electrical Engineering and Computer Science, Case Western Reserve University

A method is discussed by which the in vivo mechanical behavior of stimuli-responsive materials is monitored as a function of time. Samples are tested ex vivo using a microtensile tester with environmental controls to simulate the physiological environment. This work further promotes understanding the in vivo behavior of our material.

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Neuroscience

Fabrication of High Contact-Density, Flat-Interface Nerve Electrodes for Recording and Stimulation Applications
Yazan M. Dweiri 1,3, Matthew A. Stone 2, Dustin J. Tyler 1, Grant A. McCallum 1, Dominique M. Durand 1
1Neural Engineering Center, Case Western Reserve University, 2Advanced Platform Technology Center, U.S. Department of Veterans Affairs, 3Jordan University of Science and Technology

This article provides a detailed description on the fabrication process of a high contact-density flat interface nerve electrode (FINE). This electrode is optimized for recording and stimulating neural activity selectively within peripheral nerves.

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Bioengineering

Rodent Behavioral Testing to Assess Functional Deficits Caused by Microelectrode Implantation in the Rat Motor Cortex
Monika Goss-Varley 1,2, Andrew J. Shoffstall 1,2, Keith R. Dona 1,2, Justin A. McMahon 1,2, Sydney C. Lindner 1,2, Evon S. Ereifej 1,2, Jeffrey R. Capadona 1,2
1Advanced Platform Technology Center, Rehabilitation Research and Development, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, 2Department of Biomedical Engineering, Case Western Reserve University

We have shown that a microelectrode implantation in the motor cortex of rats causes immediate and lasting motor deficits. The methods proposed herein outline a microelectrode implantation surgery and three rodent behavioral tasks to elucidate potential changes in the fine or gross motor function due to implantation-caused damage to the motor cortex.

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Behavior

A Novel Single Animal Motor Function Tracking System Using Simple, Readily Available Software
Keith R. Dona 1,2, Monika Goss-Varley 1,2, Andrew J. Shoffstall 1,2, Jeffrey R. Capadona 1,2
1Advanced Platform Technology Center, Rehabilitation Research and Development, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, 2Department of Biomedical Engineering, Case Western Reserve University

The current study aimed to automate the quantification of motor deficits in rats. The initial evaluation model assesses motor loss resulting from an intracortical microelectrode implantation in the motor cortex. We report on the development and use of a tracking algorithm using easily adaptable, simple, and readily available coding software.

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Behavior

Characterization of the Sense of Agency over the Actions of Neural-machine Interface-operated Prostheses
Jonathon S. Schofield 1, Courtney E. Shell 1, Zachary C. Thumser 1,2, Dylan T. Beckler 1, Raviraj Nataraj 3, Paul D. Marasco 1,4
1Laboratory for Bionic Integration, Department of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic, 2Research Service, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, 3Department of Biomedical Engineering, Stevens Institute of Technology, 4Advanced Platform Technology Center, Louis Stokes Cleveland Department of Veterans Affairs Medical Center

Here we present a protocol which characterizes the sense of agency developed over the control of sensate virtual or robotic prosthetic hands. Psychophysical questionnaires are employed to capture the explicit experience of agency, and time interval estimates (intentional binding) are employed to implicitly measure the sense of agency.

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Bioengineering

Tools for Surface Treatment of Silicon Planar Intracortical Microelectrodes
Olivia K. Krebs 1,2, Gaurav Mittal 1,2, Shreya Ramani 1,2, Jichu Zhang 1,2, Andrew J. Shoffstall 1,2, Stuart F. Cogan 3, Joseph J. Pancrazio 3, Jeffrey R. Capadona 1,2
1Department of Biomedical Engineering, Case Western Reserve University, 2Advanced Platform Technology Center, Rehabilitation Research and Development, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, 3Department of Bioengineering, The University of Texas at Dallas

The present protocol describes tools for handling silicon planar intracortical microelectrodes during treatments for surface modification via gas deposition and aqueous solution reactions. The assembly of the components used to handle the devices throughout the procedure is explained in detail.

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Neuroscience

Assessment of Thermal Damage from Robot-Drilled Craniotomy for Cranial Window Surgery in Mice
George F. Hoeferlin *1,2, Dhariyat M. Menendez *1,2, Olivia K. Krebs 1,2, Jeffrey R. Capadona 1,2, Andrew J. Shoffstall 1,2
1Department of Biomedical Engineering, Case Western Reserve University, 2Advanced Platform Technology Center, Louis Stokes Cleveland VA Medical Center

Cranial windows have become a ubiquitously implemented surgical technique to allow for intravital imaging in transgenic mice. This protocol describes the use of a surgical robot that performs semi-automated bone drilling of cranial windows and can help reduce surgeon-to-surgeon variability and partially mitigate thermal blood-brain barrier damage.

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