Howard Hughes Medical Institute and Gene Expression Laboratory
4 ARTICLES PUBLISHED IN JoVE
Biology
Spinal Cord Electrophysiology
Allyn Meyer 1, Benjamin W. Gallarda 1,2, Samuel Pfaff 1, William Alaynick 1
1The Salk Institute for Biological Studies, Howard Hughes Medical Institute and Gene Expression Laboratory, 2Biology Graduate Program, University of California San Diego - UCSD
A demonstration of the isolation of neonatal mouse spinal cord for electrophysiologic studies.
Neuroscience
Spinal Cord Electrophysiology II: Extracellular Suction Electrode Fabrication
Suresh Garudadri 1, Benjamin Gallarda 1, Samuel Pfaff 1, William Alaynick 1
1Howard Hughes Medical Institute and Gene Expression Laboratory, The Salk Institute for Biological Studies
A demonstration of the fabrication and use of an extracellular suction electrode used to measure electrophysiological recordings of neonatal rodent spinal cords in vitro
Neuroscience
Using Affordable LED Arrays for Photo-Stimulation of Neurons
Matthew Valley 1, Sebastian Wagner 1, Benjamin W. Gallarda 1, Pierre-Marie Lledo 1
1Laboratory for Perception and Memory, Institut Pasteur and Centre National de la Recherche Scientifique (CNRS)
Adult-born neurons expressing ChR2 can be manipulated in slice electrophysiological preparations in order to examine their contribution towards the function of olfactory neural circuits.
Neuroscience
Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation
Gabriel Lepousez 1, Mariana Alonso 1, Sebastian Wagner 1, Benjamin W. Gallarda 1, Pierre-Marie Lledo 1
1Laboratory for Perception and Memory, Institut Pasteur and Centre National de la Recherche Scientifique (CNRS)
Adult-born neurons of the olfactory bulb can be optogenetically controlled using Channelrhodopsin2-expressing lentiviral injection in the rostral migratory stream and chronic photostimulation with an implanted miniature LED.
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