Preparation of Acute Myocardial Tissue Slices from Biopsies of Human Neonates and Infants with Congenital Heart Disease for Physiological MeasurementsMoritz Haustein 1, Rewa Nazzal 1, Jan Trieschmann 2, Gerardus Bennink 3, Juergen Hescheler 2, Frank Pillekamp 4, Tobias Hannes 1,5
1Department of Pediatric Cardiology, University Hospital of Cologne, 2Institute for Neurophysiology, University of Cologne, 3Department of Cardio-thoracic Surgery, University Hospital of Cologne, 4Department of General Pediatrics, Neonatology and Pediatric Cardiology, University Children's Hospital, Heinrich Heine University, 5Children's Hospital for Wales, University Hospital of Wales
Cardiac slices are a unique model for cardiovascular research and bridge the gap between single-cell and whole-heart models. This protocol describes the preparation of viable cardiac slices from myocardial tissue samples excised during surgery for congenital heart disease.
