Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light ScatteringDaniela Baitan 1,2, Robin Schubert 2,3, Arne Meyer 1, Karsten Dierks 1, Markus Perbandt 2,3, Christian Betzel 2,3
1Xtal Concepts GmbH, 2Institute for Biochemistry and Molecular Biology, Laboratory for Structural Biology of Infection and Inflammation c/o DESY, University of Hamburg, 3The Hamburg Center for Ultrafast Imaging, University of Hamburg
Here we present a protocol for controlled production of protein microcrystals. The process uses an automated device allowing controlled manipulation of several crystallization parameters. The protein crystallization is carried-out by controlled and automated addition of crystallization solutions while monitoring and investigating the radius distribution of particles in the crystallization droplet.
