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Preparation of Mouse Muscle Cell Suspension for Isolation of Satellite Cells by Fluorescence-Activated Cell Sorting

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03:36 min

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July 7th, 2023

DOI :

10.3791/200375-v

July 7th, 2023

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Kamar Ghaibour*¹, Joe Rizk*¹, Claudine Ebel¹, Tao Ye¹, Muriel Philipps¹, Valérie Schreiber¹, Daniel Metzger¹, Delphine Duteil¹

¹CNRS, Inserm, IGBMC UMR 7104- UMR-S 1258, Université de Strasbourg

* These authors contributed equally

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Keywords Mouse Muscle Cell Suspension

Satellite Cell Isolation

Fluorescence activated Cell Sorting

Limb Muscle Dissection

Collagenase Digestion

Red Blood Cell Lysis

Seriden

Satellite Cell Isolation from Mouse Limb Muscles and Flow Cytometry Analysis

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