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Poly(A) RNA Purification and Bisulfite Conversion for Next Generation Sequencing

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05:45 min

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July 7th, 2023

DOI :

10.3791/200389-v

July 7th, 2023

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1Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, 2Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University

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Keywords Poly A RNA Purification
Bisulfite Conversion
Next generation Sequencing
Total RNA
Oligo dT Beads
Lysis Buffer
Wash Buffer
Tris HCl
RNA Elution
Magnetic Separation
RNA Sample Preparation
RNA Enrichment

Seriden

Creating Robust Bisulfite-mRNA Libraries for Transcriptome-Wide Mapping
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