Preparation of Metabolites from a Coral Holobiont, Separated Coral Host Tissue, and Symbiodiniaceae Cells for Gas Chromatography-Mass Spectrometry Analysis
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To extract intracellular metabolites from lyophilized holobiont add 400 microliters of 100%cold methanol with internal standards to the lyophilized holobiont. Then add 10 milligrams of acid washed glass beads and place the tube in a pre chilled be
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