optimizing human intestinal enteroid cultivation for bioluminescence assays

Sirkadiyen Ritim Çalışmaları için İnsan Bağırsak Enteroidlerinin Optimize Edilmesi

Circadian clock 
All organisms, from bacteria to mammals, have a complex and dynamic relationship with their environment. Within this relationship, adaptation to environmental changes is critical for the survival of organisms. Most organisms possess circadian rhythms that enable them to adapt and optimize their functions to diurnal cycles of approximately 24 h. The circadian clock is a hierarchical network of central and peripheral clocks that work in cooperation to maintain physiological homeostasis and keep organisms synchronized with daily changes1,2. In mammals, the central or master clock located in the suprachiasmatic nucleus (SCN) receives external cues, such as light, and transmits the information to the peripheral clocks via an advanced interplay of neural and humoral signaling pathways3. In addition to the central clock, peripheral tissues possess their own cell-autonomous circadian clock mechanism, maintained by a transcriptional-translational negative feedback loop (TTFL) regulating tissue-specific clock-controlled genes (CCGs)4,5. This molecular machinery produces approximately 24 h rhythmicity in cellular and physiological events, such as gene expressions, signaling pathways, immune responses, and digestion. The circadian clock is present in almost all mammalian cells, and it has been demonstrated that up to 50% of the genes&#39; expression patterns exhibit circadian rhythmicity6. Considering the abundance of CCGs, disruption of this clock mechanism may result in critical physiological problems. Hence, investigations into circadian rhythms are necessary to elucidate essential biological mechanisms and develop novel therapeutic strategies.
Luciferase reporter system 
In circadian studies, real-time monitoring is critical for a better understanding of cellular behaviors and responses because it allows tracking of temporal changes in gene expression and/or protein levels, providing insights into the molecular mechanisms regulated by the circadian clock. Furthermore, real-time monitoring enables researchers to study the effects of environmental changes on molecular mechanisms7,8. There are numerous techniques for real-time monitoring studies, including bioluminescence assay, which is widely used to track gene expression or protein levels over time. Bioluminescence assay is a method to detect biological processes using light production as a readout. In this assay, an oxidative enzyme that produces bioluminescence (e.g., luciferase) is either transiently or stably transfected into cells of interest, and the bioluminescence readout is measured in the presence of a substrate (e.g., luciferin) over time. For example, the luciferase enzyme produces bioluminescence by oxidizing the substrate luciferin in the presence of ATP9. Due to its short half-life, 3-4 h10, firefly luciferase is a powerful tool for circadian studies in terms of providing real-time dynamic monitoring with minimal background noise11,12,13. For the insertion of DNA with a luciferase-tagged promoter or open reading frame (ORF), the lentiviral gene delivery system is a reliable method that provides high transduction efficacy, stable integration, and low immunogenicity. Stable transduction of a bioluminescent reporter provides robust expression in dividing and non-dividing cells, generating consistent data for circadian studies14.
Organoid as a model 
Traditional immortalized two-dimensional cell lines have been instrumental in biological studies ranging from uncovering fundamental molecular mechanisms of circadian rhythms to drug screening. Despite the convenience of utilizing homogenized cell lines, they lack multicellular structures and intercellular interactions. In contrast, organoids are in vitro 3D multicellular &#34;organ-like&#34; structures that mimic organ structure in a dish by displaying similarity to in vivo tissue architecture and multicellularity, including stem, progenitor, and differentiated cell types15,16. Possessing self-organization, multicellularity, and functionality features makes organoids a remarkable in vitro model representing the cellular and physiological processes occurring in real tissues17. Different types of organoids can be derived from pluripotent stem cells via directed differentiation or adult stem cells harvested from various organs, including the small intestine, brain, liver, lung, and kidney18,19. Since organoid structures possess a real tissue-like architecture and function with multicellularity and dynamic cell-to-cell interaction, they are superior to homogenized cell lines for understanding the cellular events occurring in in vivo tissues. Organoids are also easily manipulated and can be grown under controlled conditions, making them useful for circadian studies20.
The main purpose of this work is to introduce a real-time monitoring method utilizing a bioluminescence assay specifically tailored for studying circadian rhythms in multicellular 3D organoids. Real-time monitoring of cellular events using a bioluminescence assay technique has been widely performed for cell cultures lacking the multicellular complexity and intercellular communications that exist in real tissues. 3D organoids present unique opportunities to investigate the functions of circadian rhythms in multicellular systems in vitro. For example, one could investigate circadian rhythms in the organoids with altered cell compositions or organoids derived from patients&#39; diseased tissues. This protocol enables the utilization of a bioluminescence assay to investigate different aspects of circadian rhythms in a more physiologically relevant in vitro model, organoids, which will help us better understand the roles of circadian rhythms in peripheral organs.

Yazar Spotlight: Çok Hücreli Sistemlerde Sirkadiyen Ritimlerin İn Vitro Araştırmaları

Organoidlerde Biyolüminesans Raportörü ile Sirkadiyen Salınımların İzlenmesi

Our current scope of research is to investigate the roles of circadian rhythms in intestinal epithelial cell proliferation and differentiation. We are trying to determine whether the circadian clock regulates cell proliferation and differentiation from intestinal stem cells. This protocol presents a real-time monitoring method for circadian studies in three-dimensional organoids using a bioluminescence assay.Unlike performing bioluminescence assays in a conventional two-dimensional cell culture, this approach enables us to investigate circadian rhythms and their functions in a more physiologically relevant organoid model. Our findings show disrupted circadian rhythms in stem cell-enriched conditions while demonstrating robust circadian oscillations in differentiated human intestinal organoids, suggesting a remodeling of circadian clock machinery from stem cells to differentiated cells. Usages of patient-derived organoids with circadian bioluminescent reporters will help us advance our understanding of circadian rhythms in peripheral organs and different disease states.

Biyolüminesans Testleri için İnsan Bağırsağı Enteroid Yetiştiriciliğinin Optimize Edilmesi

Başlamak için, insan bağırsak enteroidlerinin veya HIE'lerin çalışma platformuna geçirilmesi için gerekli tüm malzemeleri düzenleyin. HİE'leri büyüme faktörü azaltılmış 3D kültür matrisi ile karıştırın. Karışımı, oyuk başına üç damlacık 10 mikrolitre 3D kültür matrisi içeren 24 oyuklu bir plakaya tohumlayın ve 37 santigrat derecede 20 dakika inkübe edin.Ardından 350 mikrolitre HİE büyüme ortamı ekleyin. Yedi gün sonra, HIE'leri dört kuyudan 1,5 mililitrelik tüplere toplayın. Kalıntıları temizlemek için HIE'leri 2000 g'da 40 saniye santrifüjleyin.Bir pipet kullanarak, fazla 3D kültür matrisini tüpten çıkarın. Tüpe 0,5 mililitre soğuk PBS ekleyin. Bir mililitrelik insülin şırıngası kullanarak, HIE'leri ayırmak için 10 defaya kadar şırınga ederek fiziksel türbülans uygulayın.Ayrışmış HİE'leri 2000 g'da 40 saniye santrifüjleyin ve süpernatanı nazikçe çıkarın. Ardından tüpü yaklaşık üç dakika boyunca buzun üzerine yerleştirin. Tüpe büyüme faktörü azaltılmış 3D kültür matrisi ekleyin ve pelet ile karıştırın.HİE'lerin karışımını 35 milimetrelik yuvarlak bir tabağın ortasındaki 3D kültür matrisinde tohumlayın. 3D kültür matrisini katılaştırmak için çanağı 37 santigrat derecede 20 dakika inkübe edin. Daha sonra yemeğe iki mililitre önceden ısıtılmış bağırsak organoid büyüme ortamı ekleyin ve 48 saat boyunca% 5 karbondioksit ile 37 santigrat derecede inkübe edin.Üçüncü günde, büyüme ortamını iki mililitre önceden ısıtılmış farklılaşma ortamı ile değiştirin ve 37 santigrat derecede %5 karbondioksit ile 48 saat boyunca inkübe edin. Beşinci günde, enteroidler ortamını iki mililitre önceden ısıtılmış farklılaşma ortamı ile değiştirin ve 24 saat boyunca inkübasyona devam edin.

optimizing-human-intestinal-enteroid-cultivation-for-bioluminescence

Research

JoVE Journal

Biology

Optimizing Human Intestinal Enteroid Cultivation for Bioluminescence Assays

32 Görüntüleme.  University of Cincinnati College of Medicine. Başlamak için, insan bağırsak enteroidlerinin veya HIE'lerin çalışma platformuna geçirilmesi için gerekli tüm malzemeleri düzenleyin. HİE'leri büyüme faktörü azaltılmış 3D kültür matrisi ile karıştırın. Karışımı, oyuk başına üç damlacık 10 mikrolitre 3D kültür matrisi içeren 24 oyuklu bir plakaya tohumlayın ve 37 santigrat derecede 20 dakika inkübe edin.Ardından 350 mikrolitre HİE büyüme ortamı ekleyin. Yedi gün sonra, HIE'leri dört kuyudan...

Video: Biyolüminesans Testleri için İnsan Bağırsağı Enteroid Yetiştiriciliğinin Optimize Edilmesi

Monitoring Circadian Oscillations with a Bioluminescence Reporter in Organoids

Most living organisms possess circadian rhythms, which are biological processes that occur within a period of approximately 24 h and regulate a diverse repertoire of cellular and physiological processes ranging from sleep-wake cycles to metabolism. This clock mechanism entrains the organism based on environmental changes and coordinates the temporal regulation of molecular and physiological events. Previously, it was demonstrated that autonomous circadian rhythms are maintained even at the single-cell level using cell lines such as NIH3T3 fibroblasts, which were instrumental in uncovering the mechanisms of circadian rhythms. However, these cell lines are homogeneous cultures lacking multicellularity and robust intercellular communications. In the past decade, extensive work has been performed on the development, characterization, and application of 3D organoids, which are in vitro multicellular systems that resemble in vivo morphological structures and functions. This paper describes a protocol for detecting circadian rhythms using a bioluminescent reporter in human intestinal enteroids, which enables the investigation of circadian rhythms in multicellular systems in vitro.

Circadian rhythms, which exist in most organisms, regulate the temporal organization of biological processes. 3D organoids have recently emerged as a physiologically relevant in vitro model. This protocol describes the use of bioluminescent reporters to observe circadian rhythms in organoids, enabling in vitro investigations of circadian rhythms in multicellular systems.

Most living organisms possess circadian rhythms, which are biological processes that occur within a period of approximately 24 h and regulate a diverse ...