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PCR Primer Design for Cloning of the Porcine Reproductive and Respiratory Syndrome Virus Gene

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01:58 min

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May 17th, 2024

DOI :

10.3791/201619-v

May 17th, 2024

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Ming Yang*¹^,², Lijuan Cui*³, Xuesha Liu¹

¹College of Life Sciences, China West Normal University, ²Sichuan Sports College, ³Pathology Department, Suining Central Hospital

* These authors contributed equally

Transkript

Daha Fazla Video Keşfet

PCR Primer Design

PRRSV Gene Sequence

Fragment Junctions

Specific Sequence Primers

Restriction Site

Vector Overhang Sequences

Agarose Gel Electrophoresis

XDNA Loading Buffer

Gene Amplification

Seriden

Cloning the Full-Length PRRSV Gene in the pVAX1 Expression Vector

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