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Continuous Gradient Method for Porcine Islet Purification

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38 Views

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02:22 min

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June 7th, 2024

DOI :

10.3791/201750-v

June 7th, 2024

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Sophia H. Roberts¹^,², Louai Alrata¹^,², Dahlia Abdulsattar¹^,², Karan Joseph¹^,², Wahid Abu-Amer¹^,², Mohamed Zaghloul¹^,², Batool Arif¹^,², Tarek Alhamad³, Maria S. Remedi⁴, Yiing Lin⁵, Mohamed A. Zayed¹^,²^,⁶^,⁷

¹Department of Surgery, Section of Vascular Surgery, Washington University School of Medicine, ²CardioVascular Research Innovation in Surgery and Engineering Center, Washington University School of Medicine, ³Department of Medicine, Division of Nephrology, Washington University School of Medicine, ⁴Department of Medicine, Division of Endocrinology, Washington University School of Medicine, ⁵Department of Surgery, Section of Transplant Surgery, Washington University School of Medicine, ⁶Division of Molecular Cell Biology, Washington University School of Medicine, ⁷Department of Biomedical Engineering, Washington University McKelvey School of Engineering

Transkript

Daha Fazla Video Keşfet

Continuous Gradient Method

Porcine Islet Purification

High density Gradient Solution

Purified Islet Fractions

Light Microscopy

Pancreatic Islet Isolation

Live dead Staining

Islet Size Distribution

Yield And Purity

Islet Cell Counter

Seriden

Purification of Porcine Islets by Surgical Perfusion of Pig Pancreas

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