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Assessing Epithelial Barrier Integrity of Organoid-Derived 2D Monolayers Using Paracellular Permeability Assay

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190 Views

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01:36 min

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June 14th, 2024

DOI :

10.3791/201856-v

June 14th, 2024

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Gerald D. Dykstra*¹, Minae Kawasaki*², Yoko M. Ambrosini²

¹Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, ²Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University

* These authors contributed equally

Daha Fazla Video Keşfet

Epithelial Barrier Integrity

Organoid derived 2D Monolayers

Paracellular Permeability Assay

FITC dextran Tracer

Fluorescence Intensity

Microplate Reader

Seriden

Developing 2D Monolayer Cultures from Bovine Intestinal Organoids

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