Oturum Aç

Generating Single-Cell Suspension from Lung Tissue for Identifying Rare Antigen-Specific T-Cell Populations

Please note that all translations are AI generated. Click here for the English version.

86 Views

•

03:21 min

•

July 19th, 2024

DOI :

10.3791/201919-v

July 19th, 2024

•

Daniel S. Shin¹^,²^,³, Juliana Barreto de Albuquerque¹^,³, James J. Moon¹^,³^,⁴

¹Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy, and Immunology, Massachusetts General Hospital, ²Division of Immunology, Boston Children's Hospital, ³Harvard Medical School, ⁴Division of Pulmonary and Critical Care Medicine, Massachusetts General Hospital

Transkript

Daha Fazla Video Keşfet

Single cell Suspension

Antigen specific T cell Populations

Tissue Dissociator

Liberase Stock Solution

Aminoguanidine Stock Solution

Microfuge Tubes

DNase I Digestion

Fc Block Solution

Seriden

Isolation and Characterization of Antigen-Specific T Cells in Mouse Lungs

Telif Hakkı © 2020 MyJove Corporation. Tüm hakları saklıdır