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Staining the Antigen-Specific T Cells with Peptide:MHC Tetramers for Flow Cytometry Analysis and Sorting

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01:37 min

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July 19th, 2024

DOI :

10.3791/201921-v

July 19th, 2024

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Daniel S. Shin¹^,²^,³, Juliana Barreto de Albuquerque¹^,³, James J. Moon¹^,³^,⁴

¹Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy, and Immunology, Massachusetts General Hospital, ²Division of Immunology, Boston Children's Hospital, ³Harvard Medical School, ⁴Division of Pulmonary and Critical Care Medicine, Massachusetts General Hospital

Transkript

Daha Fazla Video Keşfet

Antigen specific T Cells

Peptide MHC Tetramers

Flow Cytometry Analysis

Cell Surface Markers

Flow Cytometry Count Beads

Cold Sorter Buffer

Seriden

Isolation and Characterization of Antigen-Specific T Cells in Mouse Lungs

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