Preparation and Sorting of CT-FR-Stained Zebrafish T-ALL Cells for Transplantation and Tumor Tracking

For the preparation of the donor plate transfer 500, 000 wild type CG1 zebrafish cells into a 15 milliliter conical tube. Add five milliliters of fish media to dilute the cell stock to 100 cells per microliter. Dispense 50 microliters of the cell