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Flow Cytometric Assessment of Oxygen-Dependent CAR Expression in CAR-T Cells

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02:44 min

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June 14th, 2024

DOI :

10.3791/201981-v

June 14th, 2024

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Ying Xue*¹, Yunyu Mao*², Qibin Liao³, Chen Zhao⁴, Xiaoyan Zhang¹^,²^,⁴, Jianqing Xu¹^,²^,⁴

¹Institutes of Biomedical Sciences, Fudan University, ²Clinical Center of Biotherapy at Zhongshan Hospital, Fudan University, ³Department of Oncology and Bio-therapeutic Center, Shenzhen Third People's Hospital, Southern University of Science and Technology, ⁴Shanghai Public Health Clinical Center, Fudan University

* These authors contributed equally

Transkript

Daha Fazla Video Keşfet

Oxygen dependent CAR Expression

Phycoerythrin Conjugated Anti flag Antibody

Single Living Cells

GFP Positive Cells

PE Positive Cells

Median Fluorescence Intensity

Hypoxic Conditions

Normoxic Conditions

Seriden

Functional Validation of Hypoxia-Sensitive CAR-T Cells for Selective Tumors

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