induction of polycystic ovarian syndrome in mice using letrozole-filled mini pumps

<meta charset="utf8"></head><body>Kontrollü Letrozol Salınımı ile Farelerde Polikistik Over Sendromu İndüksiyonu

Polycystic ovary syndrome (PCOS) is one of the most common conditions among women of reproductive age1. It affects up to 18% of women globally and is the leading cause of female infertility worldwide2,3. PCOS is characterized by a series of interrelated reproductive abnormalities, including disturbed gonadotropin secretion, chronic anovulation, increased androgen production, and polycystic ovarian morphology4. In addition to gynecological disorders, PCOS also increases the risk of cardiovascular diseases5,6. Despite decades of research, the etiology of PCOS remains unclear7,8.
To gain better insights into the pathogenesis of PCOS and develop novel therapies, the creation of animal models that closely mimic human physiology is of tremendous importance9. Currently reported rodent models of PCOS include those induced by treatments with testosterone, letrozole, and estradiol valerate, among others. Testosterone induces hyperandrogenemia and is more commonly used as a PCOS inducer in rats10. DHEA has been used to induce PCOS in rodents, increasing testosterone levels, LH/FSH (luteinizing hormone/follicle-stimulating hormone) ratios, and causing irregular estrous cycles11. Estradiol valerate (EV) is a long-acting estrogen, and studies using this method show that levels of sex steroid hormones and gonadotropins vary depending on the dose of EV administered12,13,14. Letrozole is a nonsteroidal aromatase inhibitor15. Letrozole treatment induces non-cyclic estrus, increases ovarian weight, body weight, enlarges adipocytes, maximizes follicle development, and elevates testosterone levels in rats16,17. Letrozole-treated mice exhibit increased numbers of sinus follicles and hemorrhagic cysts, as well as elevated concentrations of LH, FSH, estradiol, and progesterone18,19.
Currently, the main methods for letrozole-induced PCOS modeling involve oral administration and subcutaneous injection, both of which require repeated daily dosing20,21,22. These methods are time-consuming and labor-intensive, and the repetitive administration likely causes significant stress to the animals23. Although some studies use letrozole pellets24,25, these products need to be customized and are expensive. This report describes a technique for inducing PCOS in mice using mini-pumps. This method is simple, time-saving, and uses surgical tools and equipment that are readily available in most laboratories.

<meta charset="utf8"></head><body>Yazar Spotlight: Polikistik Over Sendromunun Mekanizmalarının ve İndükleyici Modellerinin Araştırılması

Farelerde Polikistik Over Sendromunu İndüklemek İçin Modifiye Edilmiş Bir Teknik

Letrozol Dolgulu Mini Pompalar Kullanılarak Farelerde Polikistik Over Sendromunun İndüksiyonu

induction-polycystic-ovarian-syndrome-mice-using-letrozole-filled

Research

JoVE Journal

Medicine

Induction of Polycystic Ovarian Syndrome in Mice Using Letrozole-Filled Mini Pumps

29 Görüntüleme. Başlamak için, anestezi uygulanmış bir fareyi yüzüstü pozisyonda bir ameliyat plakasına sabitleyin. Boynun arka kısmının orta hattındaki cildi almak için forseps kullanın ve tüysüz taraftaki cildi tercih edin. Bir neşter ile yanal olarak 0,5 santimetre uzunluğunda bir kesi yapın.Ardından, insizyonun altındaki epidermisi açın ve cildi serbest bırakmak için bir hemostat ile erişin ve mini bir pompa için yer açın. Ardından, l...

Video: Letrozol Dolgulu Mini Pompalar Kullanılarak Farelerde Polikistik Over Sendromunun İndüksiyonu

A Modified Technique for Inducing Polycystic Ovary Syndrome in Mice

Polycystic ovary syndrome (PCOS) is one of the leading causes of infertility in women. Animal models are widely used to study the etiologic mechanisms of PCOS and for related drug development. Letrozole-induced mouse models replicate the metabolic and reproductive phenotypes of patients with PCOS. The traditional method of letrozole treatment in PCOS mice requires daily dosing over a certain period, which can be labor-intensive and cause significant stress to the mice. This study describes a simple and effective method for inducing PCOS in mice by implanting a controlled letrozole-releasing mini-pump. A mini-pump capable of stable, continuous release of a quantitative amount of letrozole was fabricated and implanted subcutaneously in mice under anesthesia. This study demonstrated that the mouse model successfully mimicked PCOS features after letrozole mini-pump implantation. The materials and equipment used in this study are readily available to most laboratories, requiring no special customization. Collectively, this article provides a unique, easy-to-perform method for inducing PCOS in mice.

This protocol introduces a technique for inducing polycystic ovary syndrome (PCOS) models in mice through controlled letrozole release using mini-pumps. Under adequate anesthesia, the mini-pump was implanted subcutaneously, and PCOS was successfully induced in the mice after a certain period of the mini-pump release.

Polycystic ovary syndrome (PCOS) is one of the leading causes of infertility in women. Animal models are widely used to study the etiologic mechanisms of ...