Cytoskeleton Staining to Analyze TNT Formation and Mitochondrial Transfer in MSC-Mito-GFP and ARPE19-Mito-RFP Co-culture

To begin, take the co-cultured MSC-Mito-GFP and ARPE19-Mito-RFP cells and remove the medium from the 24-well plates. Wash all cells once with 500 microliters per well of 4%poly formaldehyde. Add 500 microliters of fresh 4%poly formaldehyde and fix